Abstract
Proteins are some of the oldest biomolecules to be identified in palaeontological and archaeological substrates, although applications span prehistoric and historic contexts. This chapter outlines the techniques, analyses, and applications of liquid chromatography tandem mass spectrometry (LC-MS/MS)-based palaeoproteomics, with Zooarchaeology by Mass Spectrometry, immunological and amino acid techniques being covered elsewhere. It focuses on LC-MS/MS-based approaches, which have had a large impact on the fields of archaeology, cultural heritage, palaeontology, and palaeoecology, echoing its impact in fields such as the medical sciences, biochemistry, and food science. Multiple mass spectrometric approaches can be utilized to identify ancient proteins or peptides. As with other biomolecular approaches in archaeology, palaeoproteomics draws upon advances and approaches in osteoarchaeology, palaeopathology, zooarchaeology, and archaeobotany and can be connected both analytically and theoretically to other biomolecular techniques such as ancient DNA analysis, stable isotope analysis, and organic residue analysis.
| Original language | English |
|---|---|
| Title of host publication | Handbook of Archaeological Sciences |
| Publisher | Wiley-VCH Verlag |
| Pages | 501-510 |
| Number of pages | 10 |
| Volume | 1 |
| Edition | Second |
| ISBN (Electronic) | 9781119592112 |
| ISBN (Print) | 9781119592044 |
| DOIs | |
| Publication status | Published - 10 Feb 2023 |
Bibliographical note
Publisher Copyright:© 2023 John Wiley & Sons Ltd.
Keywords
- amino acid
- archaeological substrates
- biomolecular approaches
- cultural heritage
- DNA analysis
- food science
- liquid chromatography tandem
- mass spectrometry-based palaeoproteomics mass spectrometric approaches
- medical science