Assessing the Advantages, Limitations and Potential of Human Primary Prostate Epithelial Cells as a Pre-clinical Model for Prostate Cancer Research

Fiona M Frame; Amanda R Noble; Peter O'Toole; Jo Marrison; Timothy Godden; Andrew O'Brien; Norman J Maitland

doi:10.1007/978-3-030-22254-3_9

Assessing the Advantages, Limitations and Potential of Human Primary Prostate Epithelial Cells as a Pre-clinical Model for Prostate Cancer Research

Fiona M Frame, Amanda R Noble, Peter O'Toole, Jo Marrison, Timothy Godden, Andrew O'Brien, Norman J Maitland

Research output: Contribution to journal › Article › peer-review

Abstract

Choosing an appropriate cell model(s) is the first decision to be made before starting a new project or programme of study. Here, we address the rationale that can be behind this decision and we summarize the current cell models that are used to study prostate cancer. Researchers face the challenge of choosing a model that recapitulates the complexity and heterogeneity of prostate cancer. The use of primary prostate epithelial cells cultured from patient tissue is discussed, and the necessity for close clinical-academic collaboration in order to do this is highlighted. Finally, a novel quantitative phase imaging technique is described, along with the potential for cell characterization to not only include gene expression and protein markers but also morphological features, cell behaviour and kinetic activity.

Original language	English
Pages (from-to)	109-118
Number of pages	10
Journal	Advances in experimental medicine and biology
Volume	1164
DOIs	https://doi.org/10.1007/978-3-030-22254-3_9
Publication status	E-pub ahead of print - 2 Oct 2019

Bibliographical note

This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

Keywords

Cell Line
Cell Line, Tumor
Epithelial Cells/cytology
Humans
Male
Prostatic Neoplasms

Access to Document

10.1007/978-3-030-22254-3_9

Assessing the Advantages
This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.
Accepted author manuscript, 2.77 MB

Cite this

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title = "Assessing the Advantages, Limitations and Potential of Human Primary Prostate Epithelial Cells as a Pre-clinical Model for Prostate Cancer Research",

abstract = "Choosing an appropriate cell model(s) is the first decision to be made before starting a new project or programme of study. Here, we address the rationale that can be behind this decision and we summarize the current cell models that are used to study prostate cancer. Researchers face the challenge of choosing a model that recapitulates the complexity and heterogeneity of prostate cancer. The use of primary prostate epithelial cells cultured from patient tissue is discussed, and the necessity for close clinical-academic collaboration in order to do this is highlighted. Finally, a novel quantitative phase imaging technique is described, along with the potential for cell characterization to not only include gene expression and protein markers but also morphological features, cell behaviour and kinetic activity.",

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AU - Noble, Amanda R

AU - O'Toole, Peter

AU - Marrison, Jo

AU - Godden, Timothy

AU - O'Brien, Andrew

AU - Maitland, Norman J

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KW - Male

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