Assessing the Advantages, Limitations and Potential of Human Primary Prostate Epithelial Cells as a Pre-clinical Model for Prostate Cancer Research

Fiona M Frame, Amanda R Noble, Peter O'Toole, Jo Marrison, Timothy Godden, Andrew O'Brien, Norman J Maitland

Research output: Contribution to journalArticlepeer-review


Choosing an appropriate cell model(s) is the first decision to be made before starting a new project or programme of study. Here, we address the rationale that can be behind this decision and we summarize the current cell models that are used to study prostate cancer. Researchers face the challenge of choosing a model that recapitulates the complexity and heterogeneity of prostate cancer. The use of primary prostate epithelial cells cultured from patient tissue is discussed, and the necessity for close clinical-academic collaboration in order to do this is highlighted. Finally, a novel quantitative phase imaging technique is described, along with the potential for cell characterization to not only include gene expression and protein markers but also morphological features, cell behaviour and kinetic activity.

Original languageEnglish
Pages (from-to)109-118
Number of pages10
JournalAdvances in experimental medicine and biology
Publication statusE-pub ahead of print - 2 Oct 2019

Bibliographical note

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  • Cell Line
  • Cell Line, Tumor
  • Epithelial Cells/cytology
  • Humans
  • Male
  • Prostatic Neoplasms

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