Association of Vitamin D Metabolites With Embryo Development and Fertilization in Women With and Without PCOS Undergoing Subfertility Treatment

Thomas Keith Cunningham; Victoria Allgar; Soha R Dargham; Eric Kilpatrick; Thozhukat Sathyapalan; Stephen Maguiness; Haira R Mokhtar Rudin; Nour M Abdul Ghani; Aishah Latiff; Stephen L Atkin

doi:10.3389/fendo.2019.00013

Association of Vitamin D Metabolites With Embryo Development and Fertilization in Women With and Without PCOS Undergoing Subfertility Treatment

Thomas Keith Cunningham, Victoria Allgar, Soha R Dargham, Eric Kilpatrick, Thozhukat Sathyapalan, Stephen Maguiness, Haira R Mokhtar Rudin, Nour M Abdul Ghani, Aishah Latiff, Stephen L Atkin

The Hull York Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: The relationship between fertilization rates and 1,25-dihydroxyvitamin D (1,25(OH)2D3), 25-hydroxyvitamin D2 (25(OH)D2), 25-hydroxyvitamin D3 (25(OH)D3), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 25-hydroxy-3epi-Vitamin D3 (3epi25(OH)D3) concentrations in age and weight matched women with and without PCOS was studied. Methods: Fifty nine non-obese women, 29 with PCOS, and 30 non-PCOS undergoing IVF, matched for age and weight were included. Serum vitamin D metabolites were taken the menstrual cycle prior to commencing controlled ovarian hyperstimulation. Results: Vitamin D metabolites did not differ between PCOS and controls; however, 25(OH)D3 correlated with embryo fertilization rates in PCOS patients alone (p = 0.03). For all subjects, 3epi25(OH)D3 correlated with fertilization rate (p < 0.04) and negatively with HOMA-IR (p < 0.02); 25(OH)D2 correlated with cleavage rate, G3D3 and blastocyst (p < 0.05; p < 0.009; p < 0.002, respectively). 24,25(OH)2D3 correlated with AMH, antral follicle count, eggs retrieved and top quality embryos (G3D3) (p < 0.03; p < 0.003; p < 0.009; p < 0.002, respectively), and negatively with HOMA-IR (p < 0.01). 1,25(OH)2D3 did not correlate with any of the metabolic or embryo parameters. In slim PCOS, 25(OH)D3 correlated with increased fertilization rates in PCOS, but other vitamin D parameters did not differ to matched controls. Conclusion: 3epi25(OH)D3, 25(OH)D2, and 24,25(OH)2D3, but not 1,25(OH)2D3, were associated with embryo parameters suggesting that vitamin D metabolites other than 1,25(OH)2D3 are important in fertility.

Original language	English
Article number	13
Number of pages	7
Journal	Frontiers in Endocrinology
Volume	10
DOIs	https://doi.org/10.3389/fendo.2019.00013
Publication status	Published - 29 Jan 2019

Bibliographical note

© 2019 Cunningham, Allgar, Dargham, Kilpatrick, Sathyapalan,
Maguiness, Mokhtar Rudin, Abdul Ghani, Latiff and Atkin.

Access to Document

10.3389/fendo.2019.00013

Association of Vitamin D Metabolites With Embryo Development and Fertilization in Women With and Without PCOS Undergoing Subfertility Treatment
© 2019 Cunningham, Allgar, Dargham, Kilpatrick, Sathyapalan, Maguiness, Mokhtar Rudin, Abdul Ghani, Latiff and Atkin
Final published version, 556 KBLicence: CC BY

Cite this

Cunningham, T. K., Allgar, V., Dargham, S. R., Kilpatrick, E., Sathyapalan, T., Maguiness, S., Mokhtar Rudin, H. R., Abdul Ghani, N. M., Latiff, A., & Atkin, S. L. (2019). Association of Vitamin D Metabolites With Embryo Development and Fertilization in Women With and Without PCOS Undergoing Subfertility Treatment. Frontiers in Endocrinology, 10, Article 13. https://doi.org/10.3389/fendo.2019.00013

@article{bf560792810747828499992e99d5e5d7,

title = "Association of Vitamin D Metabolites With Embryo Development and Fertilization in Women With and Without PCOS Undergoing Subfertility Treatment",

abstract = "Objective: The relationship between fertilization rates and 1,25-dihydroxyvitamin D (1,25(OH)2D3), 25-hydroxyvitamin D2 (25(OH)D2), 25-hydroxyvitamin D3 (25(OH)D3), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 25-hydroxy-3epi-Vitamin D3 (3epi25(OH)D3) concentrations in age and weight matched women with and without PCOS was studied. Methods: Fifty nine non-obese women, 29 with PCOS, and 30 non-PCOS undergoing IVF, matched for age and weight were included. Serum vitamin D metabolites were taken the menstrual cycle prior to commencing controlled ovarian hyperstimulation. Results: Vitamin D metabolites did not differ between PCOS and controls; however, 25(OH)D3 correlated with embryo fertilization rates in PCOS patients alone (p = 0.03). For all subjects, 3epi25(OH)D3 correlated with fertilization rate (p < 0.04) and negatively with HOMA-IR (p < 0.02); 25(OH)D2 correlated with cleavage rate, G3D3 and blastocyst (p < 0.05; p < 0.009; p < 0.002, respectively). 24,25(OH)2D3 correlated with AMH, antral follicle count, eggs retrieved and top quality embryos (G3D3) (p < 0.03; p < 0.003; p < 0.009; p < 0.002, respectively), and negatively with HOMA-IR (p < 0.01). 1,25(OH)2D3 did not correlate with any of the metabolic or embryo parameters. In slim PCOS, 25(OH)D3 correlated with increased fertilization rates in PCOS, but other vitamin D parameters did not differ to matched controls. Conclusion: 3epi25(OH)D3, 25(OH)D2, and 24,25(OH)2D3, but not 1,25(OH)2D3, were associated with embryo parameters suggesting that vitamin D metabolites other than 1,25(OH)2D3 are important in fertility.",

author = "Cunningham, {Thomas Keith} and Victoria Allgar and Dargham, {Soha R} and Eric Kilpatrick and Thozhukat Sathyapalan and Stephen Maguiness and {Mokhtar Rudin}, {Haira R} and {Abdul Ghani}, {Nour M} and Aishah Latiff and Atkin, {Stephen L}",

note = "{\textcopyright} 2019 Cunningham, Allgar, Dargham, Kilpatrick, Sathyapalan, Maguiness, Mokhtar Rudin, Abdul Ghani, Latiff and Atkin.",

year = "2019",

month = jan,

day = "29",

doi = "10.3389/fendo.2019.00013",

language = "English",

volume = "10",

journal = "Frontiers in Endocrinology",

issn = "1664-2392",

publisher = "Frontiers Media S.A.",

}

Cunningham, TK, Allgar, V, Dargham, SR, Kilpatrick, E, Sathyapalan, T, Maguiness, S, Mokhtar Rudin, HR, Abdul Ghani, NM, Latiff, A & Atkin, SL 2019, 'Association of Vitamin D Metabolites With Embryo Development and Fertilization in Women With and Without PCOS Undergoing Subfertility Treatment', Frontiers in Endocrinology, vol. 10, 13. https://doi.org/10.3389/fendo.2019.00013

TY - JOUR

T1 - Association of Vitamin D Metabolites With Embryo Development and Fertilization in Women With and Without PCOS Undergoing Subfertility Treatment

AU - Cunningham, Thomas Keith

AU - Allgar, Victoria

AU - Dargham, Soha R

AU - Kilpatrick, Eric

AU - Sathyapalan, Thozhukat

AU - Maguiness, Stephen

AU - Mokhtar Rudin, Haira R

AU - Abdul Ghani, Nour M

AU - Latiff, Aishah

AU - Atkin, Stephen L

PY - 2019/1/29

Y1 - 2019/1/29

N2 - Objective: The relationship between fertilization rates and 1,25-dihydroxyvitamin D (1,25(OH)2D3), 25-hydroxyvitamin D2 (25(OH)D2), 25-hydroxyvitamin D3 (25(OH)D3), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 25-hydroxy-3epi-Vitamin D3 (3epi25(OH)D3) concentrations in age and weight matched women with and without PCOS was studied. Methods: Fifty nine non-obese women, 29 with PCOS, and 30 non-PCOS undergoing IVF, matched for age and weight were included. Serum vitamin D metabolites were taken the menstrual cycle prior to commencing controlled ovarian hyperstimulation. Results: Vitamin D metabolites did not differ between PCOS and controls; however, 25(OH)D3 correlated with embryo fertilization rates in PCOS patients alone (p = 0.03). For all subjects, 3epi25(OH)D3 correlated with fertilization rate (p < 0.04) and negatively with HOMA-IR (p < 0.02); 25(OH)D2 correlated with cleavage rate, G3D3 and blastocyst (p < 0.05; p < 0.009; p < 0.002, respectively). 24,25(OH)2D3 correlated with AMH, antral follicle count, eggs retrieved and top quality embryos (G3D3) (p < 0.03; p < 0.003; p < 0.009; p < 0.002, respectively), and negatively with HOMA-IR (p < 0.01). 1,25(OH)2D3 did not correlate with any of the metabolic or embryo parameters. In slim PCOS, 25(OH)D3 correlated with increased fertilization rates in PCOS, but other vitamin D parameters did not differ to matched controls. Conclusion: 3epi25(OH)D3, 25(OH)D2, and 24,25(OH)2D3, but not 1,25(OH)2D3, were associated with embryo parameters suggesting that vitamin D metabolites other than 1,25(OH)2D3 are important in fertility.

AB - Objective: The relationship between fertilization rates and 1,25-dihydroxyvitamin D (1,25(OH)2D3), 25-hydroxyvitamin D2 (25(OH)D2), 25-hydroxyvitamin D3 (25(OH)D3), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 25-hydroxy-3epi-Vitamin D3 (3epi25(OH)D3) concentrations in age and weight matched women with and without PCOS was studied. Methods: Fifty nine non-obese women, 29 with PCOS, and 30 non-PCOS undergoing IVF, matched for age and weight were included. Serum vitamin D metabolites were taken the menstrual cycle prior to commencing controlled ovarian hyperstimulation. Results: Vitamin D metabolites did not differ between PCOS and controls; however, 25(OH)D3 correlated with embryo fertilization rates in PCOS patients alone (p = 0.03). For all subjects, 3epi25(OH)D3 correlated with fertilization rate (p < 0.04) and negatively with HOMA-IR (p < 0.02); 25(OH)D2 correlated with cleavage rate, G3D3 and blastocyst (p < 0.05; p < 0.009; p < 0.002, respectively). 24,25(OH)2D3 correlated with AMH, antral follicle count, eggs retrieved and top quality embryos (G3D3) (p < 0.03; p < 0.003; p < 0.009; p < 0.002, respectively), and negatively with HOMA-IR (p < 0.01). 1,25(OH)2D3 did not correlate with any of the metabolic or embryo parameters. In slim PCOS, 25(OH)D3 correlated with increased fertilization rates in PCOS, but other vitamin D parameters did not differ to matched controls. Conclusion: 3epi25(OH)D3, 25(OH)D2, and 24,25(OH)2D3, but not 1,25(OH)2D3, were associated with embryo parameters suggesting that vitamin D metabolites other than 1,25(OH)2D3 are important in fertility.

U2 - 10.3389/fendo.2019.00013

DO - 10.3389/fendo.2019.00013

M3 - Article

C2 - 30761082

SN - 1664-2392

VL - 10

JO - Frontiers in Endocrinology

JF - Frontiers in Endocrinology

M1 - 13

ER -