Associative transcriptomics of traits in the polyploid crop species Brassica napus

Andrea Louise Harper; M. Trick; J. Higgins; F. Fraser; L. Clissold; R. Wells; C. Hattori; I. Bancroft; P. Werner

doi:10.1038/nbt.2302

Associative transcriptomics of traits in the polyploid crop species Brassica napus

Andrea Louise Harper, M. Trick, J. Higgins, F. Fraser, L. Clissold, R. Wells, C. Hattori, I. Bancroft, P. Werner

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Association genetics can quickly and efficiently delineate regions of the genome that control traits and provide markers to accelerate breeding by marker-assisted selection. But most crops are polyploid, making it difficult to identify the required markers and to assemble a genome sequence to order those markers. To circumvent this difficulty, we developed associative transcriptomics, which uses transcriptome sequencing to identify and score molecular markers representing variation in both gene sequences and gene expression, and correlate this with trait variation. Applying the method in the recently formed tetraploid crop Brassica napus, we identified genomic deletions that underlie two quantitative trait loci for glucosinolate content of seeds. The deleted regions contained orthologs of the transcription factor HAG1 (At5g61420), which controls aliphatic glucosinolate biosynthesis in Arabidopsis thaliana. This approach facilitates the application of association genetics in a broad range of crops, even those with complex genomes.

Original language	English
Pages (from-to)	798-802
Number of pages	5
Journal	Nature Biotechnology
Volume	30
Issue number	8
DOIs	https://doi.org/10.1038/nbt.2302
Publication status	Published - 1 Aug 2012

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AU - Harper, Andrea Louise

AU - Trick, M.

AU - Higgins, J.

AU - Fraser, F.

AU - Clissold, L.

AU - Wells, R.

AU - Hattori, C.

AU - Bancroft, I.

AU - Werner, P.

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Associative transcriptomics of traits in the polyploid crop species Brassica napus

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