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Associative transcriptomics of traits in the polyploid crop species Brassica napus

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Associative transcriptomics of traits in the polyploid crop species Brassica napus. / Harper, Andrea Louise; Trick, M.; Higgins, J.; Fraser, F.; Clissold, L.; Wells, R.; Hattori, C.; Bancroft, I.; Werner, P.

In: Nature Biotechnology, Vol. 30, No. 8, 01.08.2012, p. 798-802.

Research output: Contribution to journalArticle

Harvard

Harper, AL, Trick, M, Higgins, J, Fraser, F, Clissold, L, Wells, R, Hattori, C, Bancroft, I & Werner, P 2012, 'Associative transcriptomics of traits in the polyploid crop species Brassica napus', Nature Biotechnology, vol. 30, no. 8, pp. 798-802. https://doi.org/10.1038/nbt.2302

APA

Harper, A. L., Trick, M., Higgins, J., Fraser, F., Clissold, L., Wells, R., ... Werner, P. (2012). Associative transcriptomics of traits in the polyploid crop species Brassica napus. Nature Biotechnology, 30(8), 798-802. https://doi.org/10.1038/nbt.2302

Vancouver

Harper AL, Trick M, Higgins J, Fraser F, Clissold L, Wells R et al. Associative transcriptomics of traits in the polyploid crop species Brassica napus. Nature Biotechnology. 2012 Aug 1;30(8):798-802. https://doi.org/10.1038/nbt.2302

Author

Harper, Andrea Louise ; Trick, M. ; Higgins, J. ; Fraser, F. ; Clissold, L. ; Wells, R. ; Hattori, C. ; Bancroft, I. ; Werner, P. / Associative transcriptomics of traits in the polyploid crop species Brassica napus. In: Nature Biotechnology. 2012 ; Vol. 30, No. 8. pp. 798-802.

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@article{1a469434fef2493f8d426d2aad8b90e9,
title = "Associative transcriptomics of traits in the polyploid crop species Brassica napus",
abstract = "Association genetics can quickly and efficiently delineate regions of the genome that control traits and provide markers to accelerate breeding by marker-assisted selection. But most crops are polyploid, making it difficult to identify the required markers and to assemble a genome sequence to order those markers. To circumvent this difficulty, we developed associative transcriptomics, which uses transcriptome sequencing to identify and score molecular markers representing variation in both gene sequences and gene expression, and correlate this with trait variation. Applying the method in the recently formed tetraploid crop Brassica napus, we identified genomic deletions that underlie two quantitative trait loci for glucosinolate content of seeds. The deleted regions contained orthologs of the transcription factor HAG1 (At5g61420), which controls aliphatic glucosinolate biosynthesis in Arabidopsis thaliana. This approach facilitates the application of association genetics in a broad range of crops, even those with complex genomes.",
author = "Harper, {Andrea Louise} and M. Trick and J. Higgins and F. Fraser and L. Clissold and R. Wells and C. Hattori and I. Bancroft and P. Werner",
year = "2012",
month = "8",
day = "1",
doi = "10.1038/nbt.2302",
language = "English",
volume = "30",
pages = "798--802",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "8",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Associative transcriptomics of traits in the polyploid crop species Brassica napus

AU - Harper, Andrea Louise

AU - Trick, M.

AU - Higgins, J.

AU - Fraser, F.

AU - Clissold, L.

AU - Wells, R.

AU - Hattori, C.

AU - Bancroft, I.

AU - Werner, P.

PY - 2012/8/1

Y1 - 2012/8/1

N2 - Association genetics can quickly and efficiently delineate regions of the genome that control traits and provide markers to accelerate breeding by marker-assisted selection. But most crops are polyploid, making it difficult to identify the required markers and to assemble a genome sequence to order those markers. To circumvent this difficulty, we developed associative transcriptomics, which uses transcriptome sequencing to identify and score molecular markers representing variation in both gene sequences and gene expression, and correlate this with trait variation. Applying the method in the recently formed tetraploid crop Brassica napus, we identified genomic deletions that underlie two quantitative trait loci for glucosinolate content of seeds. The deleted regions contained orthologs of the transcription factor HAG1 (At5g61420), which controls aliphatic glucosinolate biosynthesis in Arabidopsis thaliana. This approach facilitates the application of association genetics in a broad range of crops, even those with complex genomes.

AB - Association genetics can quickly and efficiently delineate regions of the genome that control traits and provide markers to accelerate breeding by marker-assisted selection. But most crops are polyploid, making it difficult to identify the required markers and to assemble a genome sequence to order those markers. To circumvent this difficulty, we developed associative transcriptomics, which uses transcriptome sequencing to identify and score molecular markers representing variation in both gene sequences and gene expression, and correlate this with trait variation. Applying the method in the recently formed tetraploid crop Brassica napus, we identified genomic deletions that underlie two quantitative trait loci for glucosinolate content of seeds. The deleted regions contained orthologs of the transcription factor HAG1 (At5g61420), which controls aliphatic glucosinolate biosynthesis in Arabidopsis thaliana. This approach facilitates the application of association genetics in a broad range of crops, even those with complex genomes.

UR - http://www.scopus.com/inward/record.url?scp=84864883992&partnerID=8YFLogxK

U2 - 10.1038/nbt.2302

DO - 10.1038/nbt.2302

M3 - Article

VL - 30

SP - 798

EP - 802

JO - Nature Biotechnology

T2 - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 8

ER -