Atmospheric scanning electron microscope for correlative microscopy

TY - JOUR

T1 - Atmospheric scanning electron microscope for correlative microscopy

AU - Morrison, Ian E G

AU - Dennison, Clare L

AU - Nishiyama, Hidetoshi

AU - Suga, Mitsuo

AU - Sato, Chikara

AU - Yarwood, Andrew

AU - O'Toole, Peter J

N1 - Copyright © 2012 Elsevier Inc. All rights reserved.

PY - 2012

Y1 - 2012

N2 - The JEOL ClairScope is the first truly correlative scanning electron and optical microscope. An inverted scanning electron microscope (SEM) column allows electron images of wet samples to be obtained in ambient conditions in a biological culture dish, via a silicon nitride film window in the base. A standard inverted optical microscope positioned above the dish holder can be used to take reflected light and epifluorescence images of the same sample, under atmospheric conditions that permit biochemical modifications. For SEM, the open dish allows successive staining operations to be performed without moving the holder. The standard optical color camera used for fluorescence imaging can be exchanged for a high-sensitivity monochrome camera to detect low-intensity fluorescence signals, and also cathodoluminescence emission from nanophosphor particles. If these particles are applied to the sample at a suitable density, they can greatly assist the task of perfecting the correlation between the optical and electron images.

AB - The JEOL ClairScope is the first truly correlative scanning electron and optical microscope. An inverted scanning electron microscope (SEM) column allows electron images of wet samples to be obtained in ambient conditions in a biological culture dish, via a silicon nitride film window in the base. A standard inverted optical microscope positioned above the dish holder can be used to take reflected light and epifluorescence images of the same sample, under atmospheric conditions that permit biochemical modifications. For SEM, the open dish allows successive staining operations to be performed without moving the holder. The standard optical color camera used for fluorescence imaging can be exchanged for a high-sensitivity monochrome camera to detect low-intensity fluorescence signals, and also cathodoluminescence emission from nanophosphor particles. If these particles are applied to the sample at a suitable density, they can greatly assist the task of perfecting the correlation between the optical and electron images.

KW - Animals

KW - COS Cells

KW - Cell Culture Techniques

KW - Cell Line, Tumor

KW - Cercopithecus aethiops

KW - Chromosomes

KW - Green Fluorescent Proteins

KW - Humans

KW - Image Processing, Computer-Assisted

KW - Immunohistochemistry

KW - Membrane Microdomains

KW - Microscopy, Electron, Scanning

KW - Microscopy, Fluorescence

KW - Mycoplasma

KW - Receptors, Cell Surface

KW - Recombinant Proteins

KW - Tissue Fixation

U2 - 10.1016/B978-0-12-416026-2.00016-9

DO - 10.1016/B978-0-12-416026-2.00016-9

M3 - Article

C2 - 22857935

SN - 0091-679X

VL - 111

SP - 307

EP - 324

JO - Methods in cell biology

JF - Methods in cell biology

ER -