Abstract
To determine the role of B-cells in promoting CD8(+) T-cell-mediated beta cell destruction in chronically inflamed islets. RESEARCH DESIGN AND METHODS-RIP: TNFalpha-NOD mice were crossed to B-cell-deficient NOD mice, and diabetes development was monitored. We used in vitro antigen presentation assays and in vivo administration of bromodeoxyuridine coupled to flow cytometry assays to assess intra-islet T-cell activation in the absence or presence of B-cells. CD4(+)Foxp3(+) activity in the absence or presence of B-cells was tested using in vivo depletion techniques. Cytokine production and apoptosis assays determined the capacity of CD8(+) T-cells transform to cytotoxic T-lymphocytes (CTLs) and survive within inflamed islets in the absence or presence of B-cells.
Original language | English |
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Pages (from-to) | 909-17 |
Number of pages | 9 |
Journal | Diabetes |
Volume | 57 |
Issue number | 4 |
DOIs | |
Publication status | Published - Apr 2008 |
Keywords
- Animals
- Antigen-Presenting Cells
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- Cell Survival
- Cytokines
- Diabetes Mellitus, Type 1
- Inflammation
- Insulin-Secreting Cells
- Lymphocyte Depletion
- Mice
- Mice, Inbred NOD