Bacterial antibiotic resistance levels in agricultural soils as a result of treatment with pig slurry and detection of sulphonamide resistance genes

K G  Byrne; W  Gaze; E M H  Wellington; P  Kay; P  Blackwell; A  Boxall

Bacterial antibiotic resistance levels in agricultural soils as a result of treatment with pig slurry and detection of sulphonamide resistance genes

K G Byrne, W Gaze, E M H Wellington, P Kay, P Blackwell, A Boxall

Environment and Geography

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

An investigation was carried out to assess the environmental impact of veterinary medicines released into the environment through the spreading of pig manure slurry. Concern has been growing about the use of antibiotics in livestock husbandry and the possible selection for resistance genes in bacteria. Slurry containing antibiotics and bacteria is released into the environment and may result in the dissemination of resistance genes through horizontal gene transfer.

Three antibiotic groups were used in this study, a macrolide, sulphonamide and a tetracycline. The fate of these antibiotics when added to soil via slurry and direct application was measured over a two-year period. Samples were taken over this period and are currently being analyzed for resistant bacteria. Resistance to the above antibiotics was analyzed by targeting SulI, SulII and IntI genes. Populations of resistant bacteria were isolated and the distribution of SulI, SulII and IntI genes investigated.

Original language	English
Title of host publication	EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004
Editors	W Verstraete
Place of Publication	LEIDEN
Publisher	A A BALKEMA PUBLISHERS
Pages	615-617
Number of pages	3
ISBN (Print)	90-5809-653-X
Publication status	Published - 2004

Keywords

ESCHERICHIA-COLI
SWITZERLAND
ENVIRONMENT
INTEGRONS
PLASMIDS

Cite this

@inproceedings{d95f8b956a114d0dbc2cee70cf65a4be,

title = "Bacterial antibiotic resistance levels in agricultural soils as a result of treatment with pig slurry and detection of sulphonamide resistance genes",

abstract = "An investigation was carried out to assess the environmental impact of veterinary medicines released into the environment through the spreading of pig manure slurry. Concern has been growing about the use of antibiotics in livestock husbandry and the possible selection for resistance genes in bacteria. Slurry containing antibiotics and bacteria is released into the environment and may result in the dissemination of resistance genes through horizontal gene transfer.Three antibiotic groups were used in this study, a macrolide, sulphonamide and a tetracycline. The fate of these antibiotics when added to soil via slurry and direct application was measured over a two-year period. Samples were taken over this period and are currently being analyzed for resistant bacteria. Resistance to the above antibiotics was analyzed by targeting SulI, SulII and IntI genes. Populations of resistant bacteria were isolated and the distribution of SulI, SulII and IntI genes investigated.",

keywords = "ESCHERICHIA-COLI, SWITZERLAND, ENVIRONMENT, INTEGRONS, PLASMIDS",

author = "Byrne, {K G} and W Gaze and Wellington, {E M H} and P Kay and P Blackwell and A Boxall",

year = "2004",

language = "English",

isbn = "90-5809-653-X",

pages = "615--617",

editor = "W Verstraete",

booktitle = "EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004",

publisher = "A A BALKEMA PUBLISHERS",

}

Bacterial antibiotic resistance levels in agricultural soils as a result of treatment with pig slurry and detection of sulphonamide resistance genes. / Byrne, K G ; Gaze, W ; Wellington, E M H et al.
EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004. ed. / W Verstraete. LEIDEN: A A BALKEMA PUBLISHERS, 2004. p. 615-617.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Bacterial antibiotic resistance levels in agricultural soils as a result of treatment with pig slurry and detection of sulphonamide resistance genes

AU - Byrne, K G

AU - Gaze, W

AU - Wellington, E M H

AU - Kay, P

AU - Blackwell, P

AU - Boxall, A

PY - 2004

Y1 - 2004

N2 - An investigation was carried out to assess the environmental impact of veterinary medicines released into the environment through the spreading of pig manure slurry. Concern has been growing about the use of antibiotics in livestock husbandry and the possible selection for resistance genes in bacteria. Slurry containing antibiotics and bacteria is released into the environment and may result in the dissemination of resistance genes through horizontal gene transfer.Three antibiotic groups were used in this study, a macrolide, sulphonamide and a tetracycline. The fate of these antibiotics when added to soil via slurry and direct application was measured over a two-year period. Samples were taken over this period and are currently being analyzed for resistant bacteria. Resistance to the above antibiotics was analyzed by targeting SulI, SulII and IntI genes. Populations of resistant bacteria were isolated and the distribution of SulI, SulII and IntI genes investigated.

AB - An investigation was carried out to assess the environmental impact of veterinary medicines released into the environment through the spreading of pig manure slurry. Concern has been growing about the use of antibiotics in livestock husbandry and the possible selection for resistance genes in bacteria. Slurry containing antibiotics and bacteria is released into the environment and may result in the dissemination of resistance genes through horizontal gene transfer.Three antibiotic groups were used in this study, a macrolide, sulphonamide and a tetracycline. The fate of these antibiotics when added to soil via slurry and direct application was measured over a two-year period. Samples were taken over this period and are currently being analyzed for resistant bacteria. Resistance to the above antibiotics was analyzed by targeting SulI, SulII and IntI genes. Populations of resistant bacteria were isolated and the distribution of SulI, SulII and IntI genes investigated.

KW - ESCHERICHIA-COLI

KW - SWITZERLAND

KW - ENVIRONMENT

KW - INTEGRONS

KW - PLASMIDS

M3 - Conference contribution

SN - 90-5809-653-X

SP - 615

EP - 617

BT - EUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004

A2 - Verstraete, W

PB - A A BALKEMA PUBLISHERS

CY - LEIDEN

ER -