By the same authors

Bacterial antibiotic resistance levels in agricultural soils as a result of treatment with pig slurry and detection of sulphonamide resistance genes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Author(s)

  • K G Byrne
  • W Gaze
  • E M H Wellington
  • P Kay
  • P Blackwell
  • A Boxall

Department/unit(s)

Publication details

Title of host publicationEUROPEAN SYMPOSIUM ON ENVIRONMENTAL BIOTECHNOLOGY, ESEB 2004
DatePublished - 2004
Pages615-617
Number of pages3
PublisherA A BALKEMA PUBLISHERS
Place of PublicationLEIDEN
EditorsW Verstraete
Original languageEnglish
ISBN (Print)90-5809-653-X

Abstract

An investigation was carried out to assess the environmental impact of veterinary medicines released into the environment through the spreading of pig manure slurry. Concern has been growing about the use of antibiotics in livestock husbandry and the possible selection for resistance genes in bacteria. Slurry containing antibiotics and bacteria is released into the environment and may result in the dissemination of resistance genes through horizontal gene transfer.

Three antibiotic groups were used in this study, a macrolide, sulphonamide and a tetracycline. The fate of these antibiotics when added to soil via slurry and direct application was measured over a two-year period. Samples were taken over this period and are currently being analyzed for resistant bacteria. Resistance to the above antibiotics was analyzed by targeting SulI, SulII and IntI genes. Populations of resistant bacteria were isolated and the distribution of SulI, SulII and IntI genes investigated.

    Research areas

  • ESCHERICHIA-COLI, SWITZERLAND, ENVIRONMENT, INTEGRONS, PLASMIDS

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