By the same authors

From the same journal

Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of Tay-Sachs disease

Research output: Contribution to journalArticle


  • I Tews
  • A Perrakis
  • A Oppenheim
  • Z Dauter
  • K S Wilson
  • C E Vorgias


Publication details

JournalNature Structural Biology
DatePublished - Jul 1996
Issue number7
Number of pages11
Pages (from-to)638-648
Original languageEnglish


Chitin, the second most abundant polysaccharide on earth, is degraded by chitinases and chitobiases. The structure of Serratia marcescens chitobiase has been refined at 1.9 Angstrom resolution. The mature protein is folded into four domains and its active site is situated at the C-terminal end of the central (beta alpha)(8)-barrel. Based on the structure of the complex with the substrate disaccharide chitobiose, we propose an acid-base reaction mechanism, in which only one protein carboxylate acts as catalytic acid, while the nucleophile is the polar acetamido group of the sugar in a substrate-assisted reaction. The structural data lead to the hypothesis that the reaction proceeds with retention of anomeric configuration. The structure allows us to model the catalytic domain of the homologous hexosaminidases to give a structural rationale to pathogenic mutations that underlie Tay-Sachs and Sandhoff disease.

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations