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Bacterial β-Glucosidase Reveals the Structural and Functional Basis of Genetic Defects in Human Glucocerebrosidase 2 (GBA2)

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Author(s)

  • Ratana Charoenwattanasatien
  • Salila Pengthaisong
  • Imogen Breen
  • Risa Mutoh
  • Sompong Sansenya
  • Yanling Hua
  • Anupong Tankrathok
  • Liang Wu
  • Chomphunuch Songsiriritthigul
  • Hideaki Tanaka
  • Spencer J. Williams
  • Gideon J. Davies
  • Genji Kurisu
  • James R Ketudat Cairns

Department/unit(s)

Publication details

JournalChemical Biology
DateAccepted/In press - 26 Apr 2016
DateE-pub ahead of print - 26 Apr 2016
DatePublished (current) - 15 Jul 2016
Issue number7
Volume11
Number of pages10
Pages (from-to)1891-1900
Early online date26/04/16
Original languageEnglish

Abstract

Human glucosylcerebrosidase 2 (GBA2) of the CAZy family GH116 is responsible for the breakdown of glycosphingolipids on the cytoplasmic face of the endoplasmic reticulum and Golgi apparatus. Genetic defects in GBA2 result in spastic paraplegia and cerebellar ataxia, while cross-talk between GBA2 and GBA1 glucosylceramidases may affect Gaucher disease. Here, we report the first three-dimensional structure for any GH116 enzyme, Thermoanaerobacterium xylanolyticum TxGH116 β-glucosidase, alone and in complex with diverse ligands. These structures allow identification of the glucoside binding and active site residues, which are shown to be conserved with GBA2. Mutagenic analysis of TxGH116 and structural modeling of GBA2 provide a detailed structural and functional rationale for pathogenic missense mutations of GBA2.

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© American Chemical Society 2016. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

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