beta-Branched acyclic nucleoside analogues as inhibitors of Plasmodium falciparum dUTPase

Beatriz Baragana, Orla McCarthy, Paula Sanchez, Cristina Bosch-Navarrete, Marcel Kaiser, Reto Brun, Jean L. Whittingham, Shirley M. Roberts, Xiao-Xiong Zhou, Keith S. Wilson, Nils Gunnar Johansson, Dolores Gonzalez-Pacanowska, Ian H. Gilbert

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We report a series of beta-branched acyclic tritylated deoxyuridine analogues as inhibitors of Plasmodium falciparum deoxyuridine-5'-triphosphate nucleotidohydrolase (PfdUTPase), an enzyme involved in nucleotide metabolism that acts as first line of defence against uracil incorporation into DNA. Compounds were assayed against both PfdUTPase and intact parasites showing a correlation between enzyme inhibition and cellular assays. beta-Branched acyclic uridine analogues described here showed equal or slightly better potency and selectivity compared with previously reported analogues. The best inhibitor gave a K-i of 0.5 mu M against PfdUTPase with selectivity greater than 200-fold compared to the corresponding human enzyme and sub-micromolar growth inhibition of P. falciparum (EC50 0.6 mu M). A crystal structure of the complex of PfdUTPase with one of the inhibitors shows that this acyclic derivative binds to the active site in a similar manner to that previously reported for a tritylated cyclic deoxyuridine derivative. (C) 2011 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)2378-2391
Number of pages14
JournalBioorganic and Medicinal Chemistry
Issue number7
Publication statusPublished - 1 Apr 2011


  • Plasmodium falciparum
  • dUTPase

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