Bile canalicular dynamics in hepatocyte sandwich cultures

Raymond Reif; Johan Karlsson; Georgia Günther; Lynette Beattie; David Wrangborg; Seddik Hammad; Brigitte Begher-Tibbe; Amruta Vartak; Simone Melega; Paul M Kaye; Jan G Hengstler; Mats Jirstrand

doi:10.1007/s00204-015-1575-9

Bile canalicular dynamics in hepatocyte sandwich cultures

Raymond Reif, Johan Karlsson, Georgia Günther, Lynette Beattie, David Wrangborg, Seddik Hammad, Brigitte Begher-Tibbe, Amruta Vartak, Simone Melega, Paul M Kaye, Jan G Hengstler, Mats Jirstrand

The Hull York Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

Many substances are hepatotoxic due to their ability to cause intrahepatic cholestasis. Therefore, there is a high demand for in vitro systems for the identification of cholestatic properties of new compounds. Primary hepatocytes cultivated in collagen sandwich cultures are known to establish bile canaliculi which enclose secreted biliary components. Cholestatic compounds are mainly known to inhibit bile excretion dynamics, but may also alter canalicular volume, or hepatocellular morphology. So far, techniques to assess time-resolved morphological changes of bile canaliculi in sandwich cultures are not available. In this study, we developed an automated system that quantifies dynamics of bile canaliculi recorded in conventional time-lapse image sequences. We validated the hepatocyte sandwich culture system as an appropriate model to study bile canaliculi in vitro by showing structural similarity measured as bile canaliculi length per hepatocyte to that observed in vivo. Moreover, bile canalicular excretion kinetics of CMFDA (5-chloromethylfluorescein diacetate) in sandwich cultures resembled closely the kinetics observed in vivo. The developed quantification technique enabled the quantification of dynamic changes in individual bile canaliculi. With this technique, we were able to clearly distinguish between sandwich cultures supplemented with dexamethasone and insulin from control cultures. In conclusion, the automated quantification system offers the possibility to systematically study the causal relationship between disturbed bile canalicular dynamics and cholestasis.

Original language	English
Pages (from-to)	1861-70
Number of pages	10
Journal	Archives of toxicology
Volume	89
Issue number	10
DOIs	https://doi.org/10.1007/s00204-015-1575-9
Publication status	Published - 18 Aug 2015

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10.1007/s00204-015-1575-9

http://link.springer.com/article/10.1007%2Fs00204-015-1575-9

Cite this

@article{9433b620dc04468593ad0a95a4161249,

title = "Bile canalicular dynamics in hepatocyte sandwich cultures",

abstract = "Many substances are hepatotoxic due to their ability to cause intrahepatic cholestasis. Therefore, there is a high demand for in vitro systems for the identification of cholestatic properties of new compounds. Primary hepatocytes cultivated in collagen sandwich cultures are known to establish bile canaliculi which enclose secreted biliary components. Cholestatic compounds are mainly known to inhibit bile excretion dynamics, but may also alter canalicular volume, or hepatocellular morphology. So far, techniques to assess time-resolved morphological changes of bile canaliculi in sandwich cultures are not available. In this study, we developed an automated system that quantifies dynamics of bile canaliculi recorded in conventional time-lapse image sequences. We validated the hepatocyte sandwich culture system as an appropriate model to study bile canaliculi in vitro by showing structural similarity measured as bile canaliculi length per hepatocyte to that observed in vivo. Moreover, bile canalicular excretion kinetics of CMFDA (5-chloromethylfluorescein diacetate) in sandwich cultures resembled closely the kinetics observed in vivo. The developed quantification technique enabled the quantification of dynamic changes in individual bile canaliculi. With this technique, we were able to clearly distinguish between sandwich cultures supplemented with dexamethasone and insulin from control cultures. In conclusion, the automated quantification system offers the possibility to systematically study the causal relationship between disturbed bile canalicular dynamics and cholestasis.",

author = "Raymond Reif and Johan Karlsson and Georgia G{\"u}nther and Lynette Beattie and David Wrangborg and Seddik Hammad and Brigitte Begher-Tibbe and Amruta Vartak and Simone Melega and Kaye, {Paul M} and Hengstler, {Jan G} and Mats Jirstrand",

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doi = "10.1007/s00204-015-1575-9",

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T1 - Bile canalicular dynamics in hepatocyte sandwich cultures

AU - Reif, Raymond

AU - Karlsson, Johan

AU - Günther, Georgia

AU - Beattie, Lynette

AU - Wrangborg, David

AU - Hammad, Seddik

AU - Begher-Tibbe, Brigitte

AU - Vartak, Amruta

AU - Melega, Simone

AU - Kaye, Paul M

AU - Hengstler, Jan G

AU - Jirstrand, Mats

PY - 2015/8/18

Y1 - 2015/8/18

N2 - Many substances are hepatotoxic due to their ability to cause intrahepatic cholestasis. Therefore, there is a high demand for in vitro systems for the identification of cholestatic properties of new compounds. Primary hepatocytes cultivated in collagen sandwich cultures are known to establish bile canaliculi which enclose secreted biliary components. Cholestatic compounds are mainly known to inhibit bile excretion dynamics, but may also alter canalicular volume, or hepatocellular morphology. So far, techniques to assess time-resolved morphological changes of bile canaliculi in sandwich cultures are not available. In this study, we developed an automated system that quantifies dynamics of bile canaliculi recorded in conventional time-lapse image sequences. We validated the hepatocyte sandwich culture system as an appropriate model to study bile canaliculi in vitro by showing structural similarity measured as bile canaliculi length per hepatocyte to that observed in vivo. Moreover, bile canalicular excretion kinetics of CMFDA (5-chloromethylfluorescein diacetate) in sandwich cultures resembled closely the kinetics observed in vivo. The developed quantification technique enabled the quantification of dynamic changes in individual bile canaliculi. With this technique, we were able to clearly distinguish between sandwich cultures supplemented with dexamethasone and insulin from control cultures. In conclusion, the automated quantification system offers the possibility to systematically study the causal relationship between disturbed bile canalicular dynamics and cholestasis.

AB - Many substances are hepatotoxic due to their ability to cause intrahepatic cholestasis. Therefore, there is a high demand for in vitro systems for the identification of cholestatic properties of new compounds. Primary hepatocytes cultivated in collagen sandwich cultures are known to establish bile canaliculi which enclose secreted biliary components. Cholestatic compounds are mainly known to inhibit bile excretion dynamics, but may also alter canalicular volume, or hepatocellular morphology. So far, techniques to assess time-resolved morphological changes of bile canaliculi in sandwich cultures are not available. In this study, we developed an automated system that quantifies dynamics of bile canaliculi recorded in conventional time-lapse image sequences. We validated the hepatocyte sandwich culture system as an appropriate model to study bile canaliculi in vitro by showing structural similarity measured as bile canaliculi length per hepatocyte to that observed in vivo. Moreover, bile canalicular excretion kinetics of CMFDA (5-chloromethylfluorescein diacetate) in sandwich cultures resembled closely the kinetics observed in vivo. The developed quantification technique enabled the quantification of dynamic changes in individual bile canaliculi. With this technique, we were able to clearly distinguish between sandwich cultures supplemented with dexamethasone and insulin from control cultures. In conclusion, the automated quantification system offers the possibility to systematically study the causal relationship between disturbed bile canalicular dynamics and cholestasis.

U2 - 10.1007/s00204-015-1575-9

DO - 10.1007/s00204-015-1575-9

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C2 - 26280096

SN - 0340-5761

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