Biohydrolysis of substituted styrene oxides by Beauveria densa CMC 3240

G Grogan, C Rippe, A Willetts

Research output: Contribution to journalArticlepeer-review

Abstract

Resting whole cell suspensions of the fungus Beauveria densa CMC 3240 containing an epoxide hydrolase have been used to resolve a series of para-substituted styrene oxides, with stereoinversion of the hydrolysed epoxide enantiomer. The regio- and hence, enantioselectivity of hydrolysis is compromised where ortho- and para-methyl- and chlorostyrene oxides are substrates. Negligible activity was observed with pura-nitrostyrene oxide as substrate. The results appear to confirm a general mechanism of enzyme-catalysed acid hydrolysis for Beauvelia spp. acting on styrene oxides.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalJournal of Molecular Catalysis B : Enzymatic
Volume3
Issue number5
Publication statusPublished - 7 Aug 1997

Keywords

  • epoxide hydrolase
  • chiral epoxide
  • chiral diol
  • Beauveria spp.
  • MICROBIOLOGICAL TRANSFORMATIONS
  • EPOXIDE HYDROLYZES
  • MICROORGANISMS
  • ACCESS

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