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BODIPY probes to study peroxisome dynamics in vivo.

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JournalPlant Journal
DatePublished - May 2010
Issue number3
Volume62
Number of pages10
Pages (from-to)529-538
Original languageEnglish

Abstract

There is a continuing need for bioprobes that are target-specific and combine speed of delivery with maintenance of normal cell behaviour. Towards this end, we are developing small pro-fluorescent molecules that provide such specificity through chemical activation by biomolecules. We have generated a set of BODIPY (boron dipyrromethane) fluorophores, including one that is intrinsically non-fluorescent but on incubation with cells becomes fluorescent at its target site. Addition of these BODIPY probes to plant cells identifies peroxisomes, as verified by co-localization with an SKL-FP construct. Interestingly, in mammalian cells, co-localization with the mammalian peroxisomal marker SelectFXTM was not observed. These data suggest fundamental differences in peroxisome composition, development or function between plant and animal cells.

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