Burkholderia sabiae sp nov., isolated from root nodules of Mimosa caesalpiniifolia

Wen-Ming Chen; Sergb M. de Faria; Jui-Hsing Chou; Euan K. James; Geoffrey N. Elliott; Janet I. Sprent; Cyril Bontemps; J. Peter W. Young; Peter Vandamme

doi:10.1099/ijs.0.65816-0

Burkholderia sabiae sp nov., isolated from root nodules of Mimosa caesalpiniifolia

Wen-Ming Chen, Sergb M. de Faria, Jui-Hsing Chou, Euan K. James, Geoffrey N. Elliott, Janet I. Sprent, Cyril Bontemps, J. Peter W. Young, Peter Vandamme

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Two rhizobial strains, Br3407(T) and Br3405, were isolated from nitrogen-fixing nodules on the roots of Mimosa caesalpiniifolia, a legume tree native to Brazil. On the basis of 16S rRNA gene sequence similarities, both strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia sabiae sp. nov. is proposed. The type strain is strain Br3407(T) (=LMG 24235(T) =BCRC 17587(T)).

Original language	English
Pages (from-to)	2174-2179
Number of pages	6
Journal	International journal of systematic and evolutionary microbiology
Volume	58
Issue number	9
DOIs	https://doi.org/10.1099/ijs.0.65816-0
Publication status	Published - Sept 2008

Keywords

RALSTONIA-TAIWANENSIS
DEOXYRIBONUCLEIC-ACID
PHYLOGENETIC TREES
SOUTH-AMERICA
STRAINS
LEGUMES
CUPRIAVIDUS
SEQUENCES
BACTERIA
PHYMATUM

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10.1099/ijs.0.65816-0

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@article{63d65cd97faf49d394e3f1ac14bc29f4,

title = "Burkholderia sabiae sp nov., isolated from root nodules of Mimosa caesalpiniifolia",

abstract = "Two rhizobial strains, Br3407(T) and Br3405, were isolated from nitrogen-fixing nodules on the roots of Mimosa caesalpiniifolia, a legume tree native to Brazil. On the basis of 16S rRNA gene sequence similarities, both strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia sabiae sp. nov. is proposed. The type strain is strain Br3407(T) (=LMG 24235(T) =BCRC 17587(T)).",

keywords = "RALSTONIA-TAIWANENSIS, DEOXYRIBONUCLEIC-ACID, PHYLOGENETIC TREES, SOUTH-AMERICA, STRAINS, LEGUMES, CUPRIAVIDUS, SEQUENCES, BACTERIA, PHYMATUM",

author = "Wen-Ming Chen and {de Faria}, {Sergb M.} and Jui-Hsing Chou and James, {Euan K.} and Elliott, {Geoffrey N.} and Sprent, {Janet I.} and Cyril Bontemps and Young, {J. Peter W.} and Peter Vandamme",

year = "2008",

month = sep,

doi = "10.1099/ijs.0.65816-0",

language = "English",

volume = "58",

pages = "2174--2179",

journal = "International journal of systematic and evolutionary microbiology",

issn = "1466-5026",

publisher = "Society for General Microbiology",

number = "9",

}

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T1 - Burkholderia sabiae sp nov., isolated from root nodules of Mimosa caesalpiniifolia

AU - Chen, Wen-Ming

AU - de Faria, Sergb M.

AU - Chou, Jui-Hsing

AU - James, Euan K.

AU - Elliott, Geoffrey N.

AU - Sprent, Janet I.

AU - Bontemps, Cyril

AU - Young, J. Peter W.

AU - Vandamme, Peter

PY - 2008/9

Y1 - 2008/9

N2 - Two rhizobial strains, Br3407(T) and Br3405, were isolated from nitrogen-fixing nodules on the roots of Mimosa caesalpiniifolia, a legume tree native to Brazil. On the basis of 16S rRNA gene sequence similarities, both strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia sabiae sp. nov. is proposed. The type strain is strain Br3407(T) (=LMG 24235(T) =BCRC 17587(T)).

AB - Two rhizobial strains, Br3407(T) and Br3405, were isolated from nitrogen-fixing nodules on the roots of Mimosa caesalpiniifolia, a legume tree native to Brazil. On the basis of 16S rRNA gene sequence similarities, both strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia sabiae sp. nov. is proposed. The type strain is strain Br3407(T) (=LMG 24235(T) =BCRC 17587(T)).

KW - RALSTONIA-TAIWANENSIS

KW - DEOXYRIBONUCLEIC-ACID

KW - PHYLOGENETIC TREES

KW - SOUTH-AMERICA

KW - STRAINS

KW - LEGUMES

KW - CUPRIAVIDUS

KW - SEQUENCES

KW - BACTERIA

KW - PHYMATUM

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U2 - 10.1099/ijs.0.65816-0

DO - 10.1099/ijs.0.65816-0

M3 - Article

SN - 1466-5026

VL - 58

SP - 2174

EP - 2179

JO - International journal of systematic and evolutionary microbiology

JF - International journal of systematic and evolutionary microbiology

IS - 9

ER -

Burkholderia sabiae sp nov., isolated from root nodules of Mimosa caesalpiniifolia

Abstract

Keywords

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In Search of Beta-Rhizobia

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Burkholderia sabiae sp nov., isolated from root nodules of Mimosa caesalpiniifolia

Abstract

Keywords

Access to Document

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In Search of Beta-Rhizobia

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