TY - JOUR
T1 - C-C hydrolases for biocatalysis
AU - Siirola, E.
AU - Kroutil, W.
AU - Frank, A.
AU - Grogan, G.
PY - 2013/6/17
Y1 - 2013/6/17
N2 - Although C-C bond hydrolases are distributed widely in Nature, they has as yet have received only limited attention in the area of biocatalysis compared to their counterpart the C-heteroatom hydrolases, such as lipases and proteases. However, the substrate range of C-C hydrolases, and their non-dependence on cofactors, suggest that these enzymes may have considerable potential for applications in synthesis. In addition, hydrolases such as the β-diketone hydrolase from Rhodococcus (OCH) are known, that catalyse the formation of interesting chiral intermediates. Further enzymes, such as kynureninase and a meta-cleavage product hydrolase (MhpC), are able to catalyse carbon-carbon bond formation, suggesting wider applications in biocatalysis than previously envisaged. In this review, the distribution, catalytic characteristics and applications of C-C hydrolases are described, with a view to assessing their potentialfor use in biocatalytic processes in the future.
AB - Although C-C bond hydrolases are distributed widely in Nature, they has as yet have received only limited attention in the area of biocatalysis compared to their counterpart the C-heteroatom hydrolases, such as lipases and proteases. However, the substrate range of C-C hydrolases, and their non-dependence on cofactors, suggest that these enzymes may have considerable potential for applications in synthesis. In addition, hydrolases such as the β-diketone hydrolase from Rhodococcus (OCH) are known, that catalyse the formation of interesting chiral intermediates. Further enzymes, such as kynureninase and a meta-cleavage product hydrolase (MhpC), are able to catalyse carbon-carbon bond formation, suggesting wider applications in biocatalysis than previously envisaged. In this review, the distribution, catalytic characteristics and applications of C-C hydrolases are described, with a view to assessing their potentialfor use in biocatalytic processes in the future.
U2 - 10.1002/adsc.201300232
DO - 10.1002/adsc.201300232
M3 - Article
AN - SCOPUS:84879222671
SN - 1615-4150
VL - 355
SP - 1677
EP - 1691
JO - Advanced Synthesis and Catalysis
JF - Advanced Synthesis and Catalysis
IS - 9
ER -