Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation

Chris MacDonald; Nicholas J. Buchkovich; Daniel K. Stringer; Scott D. Emr; Robert C. Piper

doi:10.1038/embor.2012.18

Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation

Chris MacDonald, Nicholas J. Buchkovich, Daniel K. Stringer, Scott D. Emr^*, Robert C. Piper

^*Corresponding author for this work

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The efficient formation of a variety of transport vesicles is influenced by the presence of cargo, suggesting that cargo itself might have a defining role in vesicle biogenesis. However, definitive in vivo experiments supporting this concept are lacking, as it is difficult to eliminate endogenous cargo. The Endosomal Sorting Complexes Required for Transport (ESCRT) apparatus sorts ubiquitinated membrane proteins into endosomal intralumenal vesicles (ILVs) that accumulate within multivesicular bodies. Here we show that cargo ubiquitination is required for effective recruitment of the ESCRT machinery onto endosomal membranes and for the subsequent formation of ILVs.

Original language	English
Pages (from-to)	331-338
Number of pages	8
Journal	EMBO Reports
Volume	13
Issue number	4
DOIs	https://doi.org/10.1038/embor.2012.18
Publication status	Published - Apr 2012

Keywords

endosome
lysosome
multivesicular body
ubiquitin
vacuole

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10.1038/embor.2012.18

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AU - Emr, Scott D.

AU - Piper, Robert C.

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AB - The efficient formation of a variety of transport vesicles is influenced by the presence of cargo, suggesting that cargo itself might have a defining role in vesicle biogenesis. However, definitive in vivo experiments supporting this concept are lacking, as it is difficult to eliminate endogenous cargo. The Endosomal Sorting Complexes Required for Transport (ESCRT) apparatus sorts ubiquitinated membrane proteins into endosomal intralumenal vesicles (ILVs) that accumulate within multivesicular bodies. Here we show that cargo ubiquitination is required for effective recruitment of the ESCRT machinery onto endosomal membranes and for the subsequent formation of ILVs.

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KW - lysosome

KW - multivesicular body

KW - ubiquitin

KW - vacuole

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Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation

Abstract

Keywords

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