Cell wall arabinan is essential for guard cell function

S. McQueen-Mason; L. Jones; J.L. Milne; D. Ashford

doi:10.1073/pnas.1832434100

Cell wall arabinan is essential for guard cell function

S. McQueen-Mason, L. Jones, J.L. Milne, D. Ashford

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Abstract

Stomatal guard cells play a key role in the ability of plants to survive on dry land, because their movements regulate the exchange of gases and water vapor between the external environment and the interior of the plant. The walls of these cells are exceptionally strong and must undergo large and reversible deformation during stomatal opening and closing. The molecular basis of the unique strength and flexibility of guard cell walls is unknown. We show that degradation of cell wall arabinan prevents either stomatal opening or closing. This locking of guard cell wall movements can be reversed if homogalacturonan is subsequently removed from the wall. We suggest that arabinans maintain flexibility in the cell wall by preventing homogalacturonan polymers from forming tight associations.

Original language	English
Pages (from-to)	11783-11788
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	100
Issue number	20
DOIs	https://doi.org/10.1073/pnas.1832434100
Publication status	Published - 30 Sept 2003

Bibliographical note

Keywords

COMMELINA-COMMUNIS
FERULIC ACID
POLYGALACTURONASE
PECTIN
POLYSACCHARIDES
PURIFICATION
TOMATO

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10.1073/pnas.1832434100

mcqueen-masons2.pdf

http://www.pnas.org/cgi/reprint/98/20/11812

Cite this

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title = "Cell wall arabinan is essential for guard cell function",

abstract = "Stomatal guard cells play a key role in the ability of plants to survive on dry land, because their movements regulate the exchange of gases and water vapor between the external environment and the interior of the plant. The walls of these cells are exceptionally strong and must undergo large and reversible deformation during stomatal opening and closing. The molecular basis of the unique strength and flexibility of guard cell walls is unknown. We show that degradation of cell wall arabinan prevents either stomatal opening or closing. This locking of guard cell wall movements can be reversed if homogalacturonan is subsequently removed from the wall. We suggest that arabinans maintain flexibility in the cell wall by preventing homogalacturonan polymers from forming tight associations.",

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author = "S. McQueen-Mason and L. Jones and J.L. Milne and D. Ashford",

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T1 - Cell wall arabinan is essential for guard cell function

AU - McQueen-Mason, S.

AU - Jones, L.

AU - Milne, J.L.

AU - Ashford, D.

PY - 2003/9/30

Y1 - 2003/9/30

N2 - Stomatal guard cells play a key role in the ability of plants to survive on dry land, because their movements regulate the exchange of gases and water vapor between the external environment and the interior of the plant. The walls of these cells are exceptionally strong and must undergo large and reversible deformation during stomatal opening and closing. The molecular basis of the unique strength and flexibility of guard cell walls is unknown. We show that degradation of cell wall arabinan prevents either stomatal opening or closing. This locking of guard cell wall movements can be reversed if homogalacturonan is subsequently removed from the wall. We suggest that arabinans maintain flexibility in the cell wall by preventing homogalacturonan polymers from forming tight associations.

AB - Stomatal guard cells play a key role in the ability of plants to survive on dry land, because their movements regulate the exchange of gases and water vapor between the external environment and the interior of the plant. The walls of these cells are exceptionally strong and must undergo large and reversible deformation during stomatal opening and closing. The molecular basis of the unique strength and flexibility of guard cell walls is unknown. We show that degradation of cell wall arabinan prevents either stomatal opening or closing. This locking of guard cell wall movements can be reversed if homogalacturonan is subsequently removed from the wall. We suggest that arabinans maintain flexibility in the cell wall by preventing homogalacturonan polymers from forming tight associations.

KW - COMMELINA-COMMUNIS

KW - FERULIC ACID

KW - POLYGALACTURONASE

KW - PECTIN

KW - POLYSACCHARIDES

KW - PURIFICATION

KW - TOMATO

U2 - 10.1073/pnas.1832434100

DO - 10.1073/pnas.1832434100

M3 - Article

SN - 0027-8424

VL - 100

SP - 11783

EP - 11788

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 20

ER -