Characterising live cell behaviour: traditional label-free and quantitative phase imaging approaches

TY - JOUR

T1 - Characterising live cell behaviour: traditional label-free and quantitative phase imaging approaches

AU - O'Toole, Peter John

AU - Kasprowicz, Richard Mark Robert

AU - Suman, Rakesh

N1 - ©2017 The Authors.

PY - 2017/3

Y1 - 2017/3

N2 - Label-free imaging uses inherent contrast mechanisms within cells to create image contrast without introducing dyes/labels, which may confound results. Quantitative phase imaging is label-free and offers higher content and contrast compared to traditional techniques. High-contrast images facilitate generation of individual cell metrics via more robust segmentation and tracking, enabling formation of a label-free dynamic phenotype describing cell-to-cell heterogeneity and temporal changes. Compared to population-level averages, individual cell-level dynamic phenotypes have greater power to differentiate between cellular responses to treatments, which has clinical relevance e.g. in the treatment of cancer. Furthermore, as the data is obtained label-free, the same cells can be used for further assays or expansion, of potential benefit for the fields of regenerative and personalised medicine.

AB - Label-free imaging uses inherent contrast mechanisms within cells to create image contrast without introducing dyes/labels, which may confound results. Quantitative phase imaging is label-free and offers higher content and contrast compared to traditional techniques. High-contrast images facilitate generation of individual cell metrics via more robust segmentation and tracking, enabling formation of a label-free dynamic phenotype describing cell-to-cell heterogeneity and temporal changes. Compared to population-level averages, individual cell-level dynamic phenotypes have greater power to differentiate between cellular responses to treatments, which has clinical relevance e.g. in the treatment of cancer. Furthermore, as the data is obtained label-free, the same cells can be used for further assays or expansion, of potential benefit for the fields of regenerative and personalised medicine.

KW - Ptychography

KW - Live-cell imaging

KW - Quantitative phase imaging

KW - Label-free imaging;

KW - QPI

U2 - 10.1016/j.biocel.2017.01.004

DO - 10.1016/j.biocel.2017.01.004

M3 - Article

SN - 1357-2725

VL - 84

JO - International Journal of Biochemistry and Cell Biology

JF - International Journal of Biochemistry and Cell Biology

ER -