Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.: Journal of Immunology

Gavin J Wright; Holly Cherwinski; Mildred Foster-Cuevas; Gary Brooke; Michael J Puklavec; Mike Bigler; Yaoli Song; Maria Jenmalm; Dan Gorman; Terri McClanahan; Man-Ru Liu; Marion H Brown; Jonathon D Sedgwick; Joseph H Phillips; A Neil Barclay

doi:10.4049/jimmunol.171.6.3034

Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. Journal of Immunology

Gavin J Wright, Holly Cherwinski, Mildred Foster-Cuevas, Gary Brooke, Michael J Puklavec, Mike Bigler, Yaoli Song, Maria Jenmalm, Dan Gorman, Terri McClanahan, Man-Ru Liu, Marion H Brown, Jonathon D Sedgwick, Joseph H Phillips, A Neil Barclay

Research output: Contribution to journal › Article › peer-review

Abstract

CD200 (OX2) is a broadly distributed cell surface glycoprotein that interacts with a structurally related receptor (CD200R) expressed on rodent myeloid cells and is involved in regulation of macrophage function. We report the first characterization of human CD200R (hCD200R) and define its binding characteristics to hCD200. We also report the identification of a closely related gene to hCD200R, designated hCD200RLa, and four mouse CD200R-related genes (termed mCD200RLa-d). CD200, CD200R, and CD200R-related genes were closely linked in humans and mice, suggesting that these genes arose by gene duplication. The distributions of the receptor genes were determined by quantitative RT-PCR, and protein expression was confirmed by a set of novel mAbs. The distribution of mouse and human CD200R was similar, with strongest labeling of macrophages and neutrophils, but also other leukocytes, including monocytes, mast cells, and T lymphocytes. Two mCD200 receptor-like family members, designated mCD200RLa and mCD200RLb, were shown to pair with the activatory adaptor protein, DAP12, suggesting that these receptors would transmit strong activating signals in contrast to the apparent inhibitory signal delivered by triggering the CD200R. Despite substantial sequence homology with mCD200R, mCD200RLa and mCD200RLb did not bind mCD200, and presently have unknown ligands. The CD200 receptor gene family resembles the signal regulatory proteins and killer Ig-related receptors in having receptor family members with potential activatory and inhibitory functions that may play important roles in immune regulation and balance. Because manipulation of the CD200-CD200R interaction affects the outcome of rodent disease models, targeting of this pathway may have therapeutic utility.

Original language	English
Pages (from-to)	3034-3046
Number of pages	13
Journal	J Immunol
Volume	171
Issue number	6
DOIs	https://doi.org/10.4049/jimmunol.171.6.3034
Publication status	Published - 2003

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10.4049/jimmunol.171.6.3034

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Wright, G. J., Cherwinski, H., Foster-Cuevas, M., Brooke, G., Puklavec, M. J., Bigler, M., Song, Y., Jenmalm, M., Gorman, D., McClanahan, T., Liu, M.-R., Brown, M. H., Sedgwick, J. D., Phillips, J. H., & Barclay, A. N. (2003). Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. Journal of Immunology. J Immunol, 171(6), 3034-3046. https://doi.org/10.4049/jimmunol.171.6.3034

@article{b7b4a1f6f56247629affadf2f12c9abf,

title = "Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.: Journal of Immunology",

abstract = "CD200 (OX2) is a broadly distributed cell surface glycoprotein that interacts with a structurally related receptor (CD200R) expressed on rodent myeloid cells and is involved in regulation of macrophage function. We report the first characterization of human CD200R (hCD200R) and define its binding characteristics to hCD200. We also report the identification of a closely related gene to hCD200R, designated hCD200RLa, and four mouse CD200R-related genes (termed mCD200RLa-d). CD200, CD200R, and CD200R-related genes were closely linked in humans and mice, suggesting that these genes arose by gene duplication. The distributions of the receptor genes were determined by quantitative RT-PCR, and protein expression was confirmed by a set of novel mAbs. The distribution of mouse and human CD200R was similar, with strongest labeling of macrophages and neutrophils, but also other leukocytes, including monocytes, mast cells, and T lymphocytes. Two mCD200 receptor-like family members, designated mCD200RLa and mCD200RLb, were shown to pair with the activatory adaptor protein, DAP12, suggesting that these receptors would transmit strong activating signals in contrast to the apparent inhibitory signal delivered by triggering the CD200R. Despite substantial sequence homology with mCD200R, mCD200RLa and mCD200RLb did not bind mCD200, and presently have unknown ligands. The CD200 receptor gene family resembles the signal regulatory proteins and killer Ig-related receptors in having receptor family members with potential activatory and inhibitory functions that may play important roles in immune regulation and balance. Because manipulation of the CD200-CD200R interaction affects the outcome of rodent disease models, targeting of this pathway may have therapeutic utility.",

author = "Wright, {Gavin J} and Holly Cherwinski and Mildred Foster-Cuevas and Gary Brooke and Puklavec, {Michael J} and Mike Bigler and Yaoli Song and Maria Jenmalm and Dan Gorman and Terri McClanahan and Man-Ru Liu and Brown, {Marion H} and Sedgwick, {Jonathon D} and Phillips, {Joseph H} and Barclay, {A Neil}",

year = "2003",

doi = "10.4049/jimmunol.171.6.3034",

language = "English",

volume = "171",

pages = "3034--3046",

journal = "J Immunol",

number = "6",

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Wright, GJ, Cherwinski, H, Foster-Cuevas, M, Brooke, G, Puklavec, MJ, Bigler, M, Song, Y, Jenmalm, M, Gorman, D, McClanahan, T, Liu, M-R, Brown, MH, Sedgwick, JD, Phillips, JH & Barclay, AN 2003, 'Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. Journal of Immunology', J Immunol, vol. 171, no. 6, pp. 3034-3046. https://doi.org/10.4049/jimmunol.171.6.3034

TY - JOUR

T1 - Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.

T2 - Journal of Immunology

AU - Wright, Gavin J

AU - Cherwinski, Holly

AU - Foster-Cuevas, Mildred

AU - Brooke, Gary

AU - Puklavec, Michael J

AU - Bigler, Mike

AU - Song, Yaoli

AU - Jenmalm, Maria

AU - Gorman, Dan

AU - McClanahan, Terri

AU - Liu, Man-Ru

AU - Brown, Marion H

AU - Sedgwick, Jonathon D

AU - Phillips, Joseph H

AU - Barclay, A Neil

PY - 2003

Y1 - 2003

N2 - CD200 (OX2) is a broadly distributed cell surface glycoprotein that interacts with a structurally related receptor (CD200R) expressed on rodent myeloid cells and is involved in regulation of macrophage function. We report the first characterization of human CD200R (hCD200R) and define its binding characteristics to hCD200. We also report the identification of a closely related gene to hCD200R, designated hCD200RLa, and four mouse CD200R-related genes (termed mCD200RLa-d). CD200, CD200R, and CD200R-related genes were closely linked in humans and mice, suggesting that these genes arose by gene duplication. The distributions of the receptor genes were determined by quantitative RT-PCR, and protein expression was confirmed by a set of novel mAbs. The distribution of mouse and human CD200R was similar, with strongest labeling of macrophages and neutrophils, but also other leukocytes, including monocytes, mast cells, and T lymphocytes. Two mCD200 receptor-like family members, designated mCD200RLa and mCD200RLb, were shown to pair with the activatory adaptor protein, DAP12, suggesting that these receptors would transmit strong activating signals in contrast to the apparent inhibitory signal delivered by triggering the CD200R. Despite substantial sequence homology with mCD200R, mCD200RLa and mCD200RLb did not bind mCD200, and presently have unknown ligands. The CD200 receptor gene family resembles the signal regulatory proteins and killer Ig-related receptors in having receptor family members with potential activatory and inhibitory functions that may play important roles in immune regulation and balance. Because manipulation of the CD200-CD200R interaction affects the outcome of rodent disease models, targeting of this pathway may have therapeutic utility.

AB - CD200 (OX2) is a broadly distributed cell surface glycoprotein that interacts with a structurally related receptor (CD200R) expressed on rodent myeloid cells and is involved in regulation of macrophage function. We report the first characterization of human CD200R (hCD200R) and define its binding characteristics to hCD200. We also report the identification of a closely related gene to hCD200R, designated hCD200RLa, and four mouse CD200R-related genes (termed mCD200RLa-d). CD200, CD200R, and CD200R-related genes were closely linked in humans and mice, suggesting that these genes arose by gene duplication. The distributions of the receptor genes were determined by quantitative RT-PCR, and protein expression was confirmed by a set of novel mAbs. The distribution of mouse and human CD200R was similar, with strongest labeling of macrophages and neutrophils, but also other leukocytes, including monocytes, mast cells, and T lymphocytes. Two mCD200 receptor-like family members, designated mCD200RLa and mCD200RLb, were shown to pair with the activatory adaptor protein, DAP12, suggesting that these receptors would transmit strong activating signals in contrast to the apparent inhibitory signal delivered by triggering the CD200R. Despite substantial sequence homology with mCD200R, mCD200RLa and mCD200RLb did not bind mCD200, and presently have unknown ligands. The CD200 receptor gene family resembles the signal regulatory proteins and killer Ig-related receptors in having receptor family members with potential activatory and inhibitory functions that may play important roles in immune regulation and balance. Because manipulation of the CD200-CD200R interaction affects the outcome of rodent disease models, targeting of this pathway may have therapeutic utility.

U2 - 10.4049/jimmunol.171.6.3034

DO - 10.4049/jimmunol.171.6.3034

M3 - Article

VL - 171

SP - 3034

EP - 3046

JO - J Immunol

JF - J Immunol

IS - 6

ER -