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Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae

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Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae. / Deniaud-Bouët, Estelle; Kervarec, Nelly; Michel, Gurvan; Tonon, Thierry; Kloareg, Bernard; Hervé, Cécile.

In: Annals of Botany, Vol. 114, No. 6, 29.05.2014, p. 1203-1216.

Research output: Contribution to journal › Article › peer-review

Harvard

Deniaud-Bouët, E, Kervarec, N, Michel, G, Tonon, T, Kloareg, B & Hervé, C 2014, 'Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae', Annals of Botany, vol. 114, no. 6, pp. 1203-1216. https://doi.org/10.1093/aob/mcu096

APA

Deniaud-Bouët, E., Kervarec, N., Michel, G., Tonon, T., Kloareg, B., & Hervé, C. (2014). Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae. Annals of Botany, 114(6), 1203-1216. https://doi.org/10.1093/aob/mcu096

Vancouver

Deniaud-Bouët E, Kervarec N, Michel G, Tonon T, Kloareg B, Hervé C. Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae. Annals of Botany. 2014 May 29;114(6):1203-1216. https://doi.org/10.1093/aob/mcu096

Author

Deniaud-Bouët, Estelle ; Kervarec, Nelly ; Michel, Gurvan ; Tonon, Thierry ; Kloareg, Bernard ; Hervé, Cécile. / Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae. In: Annals of Botany. 2014 ; Vol. 114, No. 6. pp. 1203-1216.

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@article{bb54864caa2844f19f76cd6bc9b5f258,
title = "Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae",
abstract = "BACKGROUND AND AIMS: Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall. They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates). How these components are organized into a three-dimensional extracellular matrix (ECM) still remains unclear. Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.METHODS: Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed. Dedicated enzymatic degradations were used to release and identify cell wall partners. This approach was complemented by systematic chromatographic analysis to study polymer interlinks further. An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made.KEY RESULTS: The data indicate that FCSPs are tightly associated with proteins and cellulose within the walls. Alginates are associated with most phenolic compounds. The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.CONCLUSIONS: The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components. Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress. The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.",
keywords = "Biological Evolution, Cell Wall/chemistry, Cellulose/metabolism, Extracellular Matrix/metabolism, Fucose/metabolism, Models, Structural, Phaeophyta/chemistry, Polysaccharides/metabolism",
author = "Estelle Deniaud-Bou{\"e}t and Nelly Kervarec and Gurvan Michel and Thierry Tonon and Bernard Kloareg and C{\'e}cile Herv{\'e}",
note = "{\textcopyright} The Author 2014. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.",
year = "2014",
month = may,
day = "29",
doi = "10.1093/aob/mcu096",
language = "English",
volume = "114",
pages = "1203--1216",
journal = "Annals of Botany",
issn = "0305-7364",
publisher = "Oxford University Press",
number = "6",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae

AU - Deniaud-Bouët, Estelle

AU - Kervarec, Nelly

AU - Michel, Gurvan

AU - Tonon, Thierry

AU - Kloareg, Bernard

AU - Hervé, Cécile

N1 - © The Author 2014. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

PY - 2014/5/29

Y1 - 2014/5/29

N2 - BACKGROUND AND AIMS: Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall. They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates). How these components are organized into a three-dimensional extracellular matrix (ECM) still remains unclear. Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.METHODS: Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed. Dedicated enzymatic degradations were used to release and identify cell wall partners. This approach was complemented by systematic chromatographic analysis to study polymer interlinks further. An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made.KEY RESULTS: The data indicate that FCSPs are tightly associated with proteins and cellulose within the walls. Alginates are associated with most phenolic compounds. The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.CONCLUSIONS: The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components. Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress. The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.

AB - BACKGROUND AND AIMS: Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall. They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates). How these components are organized into a three-dimensional extracellular matrix (ECM) still remains unclear. Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.METHODS: Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed. Dedicated enzymatic degradations were used to release and identify cell wall partners. This approach was complemented by systematic chromatographic analysis to study polymer interlinks further. An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made.KEY RESULTS: The data indicate that FCSPs are tightly associated with proteins and cellulose within the walls. Alginates are associated with most phenolic compounds. The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.CONCLUSIONS: The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components. Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress. The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.

KW - Biological Evolution

KW - Cell Wall/chemistry

KW - Cellulose/metabolism

KW - Extracellular Matrix/metabolism

KW - Fucose/metabolism

KW - Models, Structural

KW - Phaeophyta/chemistry

KW - Polysaccharides/metabolism

U2 - 10.1093/aob/mcu096

DO - 10.1093/aob/mcu096

M3 - Article

C2 - 24875633

VL - 114

SP - 1203

EP - 1216

JO - Annals of Botany

JF - Annals of Botany

SN - 0305-7364

IS - 6

ER -

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