Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis

E J  Taylor; S J  Charnock; J  Colby; G J  Davies; G W  Black

Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis

E J Taylor, S J Charnock, J Colby, G J Davies, G W Black

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 Angstrom using an in-house Cu K alpha source and belong to either space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 72.4, c = 241.2 Angstrom, and have two molecules of YckF in the asymmetric unit.

Original language	English
Pages (from-to)	1138-1140
Number of pages	3
Journal	Acta Crystallographica. Section D, Biological Crystallography
Volume	57
Publication status	Published - Aug 2001

Keywords

RIBULOSE MONOPHOSPHATE PATHWAY
METHYLOCOCCUS-CAPSULATUS
METHYLOTROPHIC BACTERIUM
SYNTHASE
GROWTH

Cite this

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title = "Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis",

abstract = "The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 Angstrom using an in-house Cu K alpha source and belong to either space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 72.4, c = 241.2 Angstrom, and have two molecules of YckF in the asymmetric unit.",

keywords = "RIBULOSE MONOPHOSPHATE PATHWAY, METHYLOCOCCUS-CAPSULATUS, METHYLOTROPHIC BACTERIUM, SYNTHASE, GROWTH",

author = "Taylor, {E J} and Charnock, {S J} and J Colby and Davies, {G J} and Black, {G W}",

year = "2001",

month = aug,

language = "English",

volume = "57",

pages = "1138--1140",

journal = "Acta Crystallographica. Section D, Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

}

TY - JOUR

T1 - Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis

AU - Taylor, E J

AU - Charnock, S J

AU - Colby, J

AU - Davies, G J

AU - Black, G W

PY - 2001/8

Y1 - 2001/8

N2 - The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 Angstrom using an in-house Cu K alpha source and belong to either space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 72.4, c = 241.2 Angstrom, and have two molecules of YckF in the asymmetric unit.

AB - The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 Angstrom using an in-house Cu K alpha source and belong to either space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 72.4, c = 241.2 Angstrom, and have two molecules of YckF in the asymmetric unit.

KW - RIBULOSE MONOPHOSPHATE PATHWAY

KW - METHYLOCOCCUS-CAPSULATUS

KW - METHYLOTROPHIC BACTERIUM

KW - SYNTHASE

KW - GROWTH

M3 - Article

SN - 0907-4449

VL - 57

SP - 1138

EP - 1140

JO - Acta Crystallographica. Section D, Biological Crystallography

JF - Acta Crystallographica. Section D, Biological Crystallography

ER -