By the same authors

From the same journal

From the same journal

Cloning, sequencing, and characterization of the hexahydro-1,3,5-trinitro-1,3,5-triazine degradation gene cluster from Rhodococcus rhodochrous

Research output: Contribution to journalArticle

Full text download(s)

Links

Published copy (DOI)

Author(s)

  • H M B Seth-Smith
  • S J Rosser
  • A Basran
  • E R Travis
  • E R Dabbs
  • S Nicklin
  • N C Bruce

Department/unit(s)

Publication details

JournalApplied and Environmental Microbiology
DatePublished - Oct 2002
Issue number10
Volume68
Number of pages8
Pages (from-to)4764-4771
Original languageEnglish

Abstract

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a high explosive which presents an environmental hazard as a major land and groundwater contaminant. Rhodococcus rhodochrous strain 11Y was isolated from explosive contaminated land and is capable of degrading RDX when provided as the sole source of nitrogen for growth. Products of RDX degradation in resting-cell incubations were analyzed and found to include nitrite, formaldehyde, and formate. No ammonium was excreted into the medium, and no dead-end metabolites were observed. The gene responsible for the degradation of RDX in strain 11Y is a constitutively expressed cytochrome P450-like gene, xpLA, which is found in a gene cluster with an adrenodoxin reductase homologue, xplB. The cytochrome P450 also has a flavodoxin domain at the N terminus. This study is the first to present a gene which has been identified as being responsible for RDX biodegradation. The mechanism of action of XplA on RDX is thought to involve initial denitration followed by spontaneous ring cleavage and mineralization.

Bibliographical note

Copyright © 2002 American Society for Microbiology

    Research areas

  • HERBICIDE-INDUCIBLE CYTOCHROMES-P-450, RDX HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE, PHANEROCHAETE-CHRYSOSPORIUM, TNT 2,4,6-TRINITROTOLUENE, STREPTOMYCES-GRISEOLUS, ADRENODOXIN REDUCTASE, BIODEGRADATION, FLAVODOXIN, OPERON, STRAIN

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations