Co-Amplification of cytochrome b and d-loop mtDNA fragments for the identification of degraded DNA samples

Dongya Yang; Camilla Filomena Speller

Co-Amplification of cytochrome b and d-loop mtDNA fragments for the identification of degraded DNA samples

Archaeology

Research output: Contribution to journal › Article › peer-review

Abstract

We propose a simple and effective approach to simultaneously co-amplify both cytochrome b and D-loop fragments to evaluate DNA preservation and to monitor possible contaminations in the analysis of degraded animal DNA samples. We have applied this approach to over 200 ancient salmon samples and 25 ancient whale DNA samples, clearly demonstrating its multiple benefits for analysis of degraded DNA samples, and the ease in which co-amplification can be optimized for different taxa. This simple, cost-efficient and genomic DNA-saving approach can be used routinely in the analysis of minute and degraded DNA samples in wildlife forensics, food inspection, conservation biology and ancient faunal remains.

Original language	English
Pages (from-to)	605-608
Number of pages	4
Journal	Molecular ecology notes
Volume	6
Issue number	3
Early online date	6 Jun 2006
Publication status	Published - Sept 2006

Keywords

animal mtDNA
cytochrome b
D-loop
PCR amplification
species identification

Cite this

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title = "Co-Amplification of cytochrome b and d-loop mtDNA fragments for the identification of degraded DNA samples",

abstract = "We propose a simple and effective approach to simultaneously co-amplify both cytochrome b and D-loop fragments to evaluate DNA preservation and to monitor possible contaminations in the analysis of degraded animal DNA samples. We have applied this approach to over 200 ancient salmon samples and 25 ancient whale DNA samples, clearly demonstrating its multiple benefits for analysis of degraded DNA samples, and the ease in which co-amplification can be optimized for different taxa. This simple, cost-efficient and genomic DNA-saving approach can be used routinely in the analysis of minute and degraded DNA samples in wildlife forensics, food inspection, conservation biology and ancient faunal remains.",

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author = "Dongya Yang and Speller, {Camilla Filomena}",

year = "2006",

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language = "English",

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pages = "605--608",

journal = "Molecular ecology notes",

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T1 - Co-Amplification of cytochrome b and d-loop mtDNA fragments for the identification of degraded DNA samples

AU - Yang, Dongya

AU - Speller, Camilla Filomena

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N2 - We propose a simple and effective approach to simultaneously co-amplify both cytochrome b and D-loop fragments to evaluate DNA preservation and to monitor possible contaminations in the analysis of degraded animal DNA samples. We have applied this approach to over 200 ancient salmon samples and 25 ancient whale DNA samples, clearly demonstrating its multiple benefits for analysis of degraded DNA samples, and the ease in which co-amplification can be optimized for different taxa. This simple, cost-efficient and genomic DNA-saving approach can be used routinely in the analysis of minute and degraded DNA samples in wildlife forensics, food inspection, conservation biology and ancient faunal remains.

AB - We propose a simple and effective approach to simultaneously co-amplify both cytochrome b and D-loop fragments to evaluate DNA preservation and to monitor possible contaminations in the analysis of degraded animal DNA samples. We have applied this approach to over 200 ancient salmon samples and 25 ancient whale DNA samples, clearly demonstrating its multiple benefits for analysis of degraded DNA samples, and the ease in which co-amplification can be optimized for different taxa. This simple, cost-efficient and genomic DNA-saving approach can be used routinely in the analysis of minute and degraded DNA samples in wildlife forensics, food inspection, conservation biology and ancient faunal remains.

KW - animal mtDNA

KW - cytochrome b

KW - D-loop

KW - PCR amplification

KW - species identification

M3 - Article

SN - 1471-8278

VL - 6

SP - 605

EP - 608

JO - Molecular ecology notes

JF - Molecular ecology notes

IS - 3

ER -