Co-existing grass species have distinctive arbuscular mycorrhizal communities

P Vandenkoornhuyse; K P Ridgway; I J Watson; A H Fitter; J P W Young

doi:10.1046/j.1365-294X.2003.01967.x

Co-existing grass species have distinctive arbuscular mycorrhizal communities

P Vandenkoornhuyse, K P Ridgway, I J Watson, A H Fitter, J P W Young

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Arbuscular mycorrhizal (AM) fungi are biotrophic symbionts colonizing the majority of land plants, and are of major importance in plant nutrient supply. Their diversity is suggested to be an important determinant of plant community structure, but the influence of host-plant and environmental factors on AM fungal community in plant roots is poorly documented. Using the terminal restriction fragment length polymorphism (T-RFLP) strategy, the diversity of AM fungi was assessed in 89 roots of three grass species (Agrostis capillaris, Festuca rubra, Poa pratensis) that co-occurred in the same plots of a field experiment. The impact of different soil amendments (nitrogen, lime, nitrogen and lime) and insecticide application on AM fungal community was also studied. The level of diversity found in AM fungal communities using the T-RFLP strategy was consistent with previous studies based on clone libraries. Our results clearly confirm that an AM fungal host-plant preference exists, even between different grass species. AM communities colonizing A. capillaris were statistically different from the others (P < 0.05). Although grass species evenness changed in amended soils, AM fungal community composition in roots of a given grass species remained stable. Conversely, in plots where insecticide was applied, we found higher AM fungal diversity and, in F. rubra roots, a statistically different AM fungal community.

Original language	English
Pages (from-to)	3085-3095
Number of pages	11
Journal	Molecular Ecology
Volume	12
Issue number	11
DOIs	https://doi.org/10.1046/j.1365-294X.2003.01967.x
Publication status	Published - Nov 2003

Bibliographical note

© 2003 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=0962-1083 or www.blackwell-synergy.com

Keywords

AM fungi
host-plant preference
soil treatment effect
SSU rRNA gene
T-RFLP
trnL intron
16S RIBOSOMAL-RNA
LENGTH-POLYMORPHISM ANALYSIS
BACTERIAL COMMUNITIES
MOLECULAR-IDENTIFICATION
FUNGAL DIVERSITY
MARINE-SEDIMENTS
GLOMUS-MOSSEAE
PLANT-ROOTS
SOIL
PHYLOGENY

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Cite this

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title = "Co-existing grass species have distinctive arbuscular mycorrhizal communities",

abstract = "Arbuscular mycorrhizal (AM) fungi are biotrophic symbionts colonizing the majority of land plants, and are of major importance in plant nutrient supply. Their diversity is suggested to be an important determinant of plant community structure, but the influence of host-plant and environmental factors on AM fungal community in plant roots is poorly documented. Using the terminal restriction fragment length polymorphism (T-RFLP) strategy, the diversity of AM fungi was assessed in 89 roots of three grass species (Agrostis capillaris, Festuca rubra, Poa pratensis) that co-occurred in the same plots of a field experiment. The impact of different soil amendments (nitrogen, lime, nitrogen and lime) and insecticide application on AM fungal community was also studied. The level of diversity found in AM fungal communities using the T-RFLP strategy was consistent with previous studies based on clone libraries. Our results clearly confirm that an AM fungal host-plant preference exists, even between different grass species. AM communities colonizing A. capillaris were statistically different from the others (P < 0.05). Although grass species evenness changed in amended soils, AM fungal community composition in roots of a given grass species remained stable. Conversely, in plots where insecticide was applied, we found higher AM fungal diversity and, in F. rubra roots, a statistically different AM fungal community.",

keywords = "AM fungi, host-plant preference, soil treatment effect, SSU rRNA gene, T-RFLP, trnL intron, 16S RIBOSOMAL-RNA, LENGTH-POLYMORPHISM ANALYSIS, BACTERIAL COMMUNITIES, MOLECULAR-IDENTIFICATION, FUNGAL DIVERSITY, MARINE-SEDIMENTS, GLOMUS-MOSSEAE, PLANT-ROOTS, SOIL, PHYLOGENY",

author = "P Vandenkoornhuyse and Ridgway, {K P} and Watson, {I J} and Fitter, {A H} and Young, {J P W}",

note = "{\textcopyright} 2003 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=0962-1083 or www.blackwell-synergy.com",

year = "2003",

month = nov,

doi = "10.1046/j.1365-294X.2003.01967.x",

language = "English",

volume = "12",

pages = "3085--3095",

journal = "Molecular Ecology",

issn = "0962-1083",

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T1 - Co-existing grass species have distinctive arbuscular mycorrhizal communities

AU - Vandenkoornhuyse, P

AU - Ridgway, K P

AU - Watson, I J

AU - Fitter, A H

AU - Young, J P W

N1 - © 2003 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=0962-1083 or www.blackwell-synergy.com

PY - 2003/11

Y1 - 2003/11

N2 - Arbuscular mycorrhizal (AM) fungi are biotrophic symbionts colonizing the majority of land plants, and are of major importance in plant nutrient supply. Their diversity is suggested to be an important determinant of plant community structure, but the influence of host-plant and environmental factors on AM fungal community in plant roots is poorly documented. Using the terminal restriction fragment length polymorphism (T-RFLP) strategy, the diversity of AM fungi was assessed in 89 roots of three grass species (Agrostis capillaris, Festuca rubra, Poa pratensis) that co-occurred in the same plots of a field experiment. The impact of different soil amendments (nitrogen, lime, nitrogen and lime) and insecticide application on AM fungal community was also studied. The level of diversity found in AM fungal communities using the T-RFLP strategy was consistent with previous studies based on clone libraries. Our results clearly confirm that an AM fungal host-plant preference exists, even between different grass species. AM communities colonizing A. capillaris were statistically different from the others (P < 0.05). Although grass species evenness changed in amended soils, AM fungal community composition in roots of a given grass species remained stable. Conversely, in plots where insecticide was applied, we found higher AM fungal diversity and, in F. rubra roots, a statistically different AM fungal community.

AB - Arbuscular mycorrhizal (AM) fungi are biotrophic symbionts colonizing the majority of land plants, and are of major importance in plant nutrient supply. Their diversity is suggested to be an important determinant of plant community structure, but the influence of host-plant and environmental factors on AM fungal community in plant roots is poorly documented. Using the terminal restriction fragment length polymorphism (T-RFLP) strategy, the diversity of AM fungi was assessed in 89 roots of three grass species (Agrostis capillaris, Festuca rubra, Poa pratensis) that co-occurred in the same plots of a field experiment. The impact of different soil amendments (nitrogen, lime, nitrogen and lime) and insecticide application on AM fungal community was also studied. The level of diversity found in AM fungal communities using the T-RFLP strategy was consistent with previous studies based on clone libraries. Our results clearly confirm that an AM fungal host-plant preference exists, even between different grass species. AM communities colonizing A. capillaris were statistically different from the others (P < 0.05). Although grass species evenness changed in amended soils, AM fungal community composition in roots of a given grass species remained stable. Conversely, in plots where insecticide was applied, we found higher AM fungal diversity and, in F. rubra roots, a statistically different AM fungal community.

KW - AM fungi

KW - host-plant preference

KW - soil treatment effect

KW - SSU rRNA gene

KW - T-RFLP

KW - trnL intron

KW - 16S RIBOSOMAL-RNA

KW - LENGTH-POLYMORPHISM ANALYSIS

KW - BACTERIAL COMMUNITIES

KW - MOLECULAR-IDENTIFICATION

KW - FUNGAL DIVERSITY

KW - MARINE-SEDIMENTS

KW - GLOMUS-MOSSEAE

KW - PLANT-ROOTS

KW - SOIL

KW - PHYLOGENY

U2 - 10.1046/j.1365-294X.2003.01967.x

DO - 10.1046/j.1365-294X.2003.01967.x

M3 - Article

SN - 0962-1083

VL - 12

SP - 3085

EP - 3095

JO - Molecular Ecology

JF - Molecular Ecology

IS - 11

ER -