COG complexes form spatial landmarks for distinct SNARE complexes

TY - JOUR

T1 - COG complexes form spatial landmarks for distinct SNARE complexes

AU - Willett, Rose

AU - Kudlyk, Tetyana

AU - Pokrovskaya, Irina

AU - Schönherr, Robert

AU - Ungar, Daniel

AU - Duden, Rainer

AU - Lupashin, Vladimir

PY - 2013/3/5

Y1 - 2013/3/5

N2 - Vesicular tethers and SNAREs (soluble N-ethylmalemide-sensitive fusion attachment protein receptors) are two key protein components of the intracellular membrane-trafficking machinery. The conserved oligomeric Golgi (COG) complex has been implicated in the tethering of retrograde intra-Golgi vesicles. Here, using yeast two-hybrid and co-immunoprecipitation approaches, we show that three COG subunits, namely COG4, 6 and 8, are capable of interacting with defined Golgi SNAREs, namely STX5, STX6, STX16, GS27 and SNAP29. Comparative analysis of COG8-STX16 and COG4-STX5 interactions by a COG-based mitochondrial relocalization assay reveals that the COG8 and COG4 proteins initiate the formation of two different tethering platforms that can facilitate the redirection of two populations of Golgi transport intermediates to the mitochondrial vicinity. Our results uncover a role for COG sub-complexes in defining the specificity of vesicular sorting within the Golgi.

AB - Vesicular tethers and SNAREs (soluble N-ethylmalemide-sensitive fusion attachment protein receptors) are two key protein components of the intracellular membrane-trafficking machinery. The conserved oligomeric Golgi (COG) complex has been implicated in the tethering of retrograde intra-Golgi vesicles. Here, using yeast two-hybrid and co-immunoprecipitation approaches, we show that three COG subunits, namely COG4, 6 and 8, are capable of interacting with defined Golgi SNAREs, namely STX5, STX6, STX16, GS27 and SNAP29. Comparative analysis of COG8-STX16 and COG4-STX5 interactions by a COG-based mitochondrial relocalization assay reveals that the COG8 and COG4 proteins initiate the formation of two different tethering platforms that can facilitate the redirection of two populations of Golgi transport intermediates to the mitochondrial vicinity. Our results uncover a role for COG sub-complexes in defining the specificity of vesicular sorting within the Golgi.

UR - http://www.scopus.com/inward/record.url?scp=84875886094&partnerID=8YFLogxK

U2 - 10.1038/ncomms2535

DO - 10.1038/ncomms2535

M3 - Article

C2 - 23462996

SN - 2041-1723

VL - 4

JO - Nature Communications

JF - Nature Communications

M1 - 1553

ER -