Community-developed checklists for publishing images and image analyses

TY - JOUR

T1 - Community-developed checklists for publishing images and image analyses

AU - QUAREP-LiMi Working Group 12

AU - Schmied, Christopher

AU - Nelson, Michael S

AU - Avilov, Sergiy

AU - Bakker, Gert-Jan

AU - Bertocchi, Cristina

AU - Bischof, Johanna

AU - Boehm, Ulrike

AU - Brocher, Jan

AU - Carvalho, Mariana T

AU - Chiritescu, Catalin

AU - Christopher, Jana

AU - Cimini, Beth A

AU - Conde-Sousa, Eduardo

AU - Ebner, Michael

AU - Ecker, Rupert

AU - Eliceiri, Kevin

AU - Fernandez-Rodriguez, Julia

AU - Gaudreault, Nathalie

AU - Gelman, Laurent

AU - Grunwald, David

AU - Gu, Tingting

AU - Halidi, Nadia

AU - Hammer, Mathias

AU - Hartley, Matthew

AU - Held, Marie

AU - Jug, Florian

AU - Kapoor, Varun

AU - Koksoy, Ayse Aslihan

AU - Lacoste, Judith

AU - Le Dévédec, Sylvia

AU - Le Guyader, Sylvie

AU - Liu, Penghuan

AU - Martins, Gabriel G

AU - Mathur, Aastha

AU - Miura, Kota

AU - Montero Llopis, Paula

AU - Nitschke, Roland

AU - North, Alison

AU - Parslow, Adam C

AU - Payne-Dwyer, Alex

AU - Plantard, Laure

AU - Ali, Rizwan

AU - Schroth-Diez, Britta

AU - Schütz, Lucas

AU - Scott, Ryan T

AU - Seitz, Arne

AU - Selchow, Olaf

AU - Sharma, Ved P

AU - Spitaler, Martin

AU - Srinivasan, Sathya

N1 - © 2023. Springer Nature America, Inc.

PY - 2023/9/14

Y1 - 2023/9/14

N2 - Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.

AB - Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.

U2 - 10.1038/s41592-023-01987-9

DO - 10.1038/s41592-023-01987-9

M3 - Review article

C2 - 37710020

SN - 1548-7091

VL - 21

JO - Nature Methods

JF - Nature Methods

ER -