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From the same journal

Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein–Ligand Interactions

Research output: Contribution to journalArticle

Author(s)

  • Yuan Guy
  • Chadmamas Sakonsinsiri
  • Inga Nehlmeier
  • Martin Anthony Fascione
  • Haiyan Zhang
  • Weili Wang
  • Stefan Pohlmann
  • W. Bruce Turnbull
  • Dejian Zhou

Department/unit(s)

Publication details

JournalAngewandte Chemie International Edition
DateAccepted/In press - 15 Mar 2016
DateE-pub ahead of print - 16 Mar 2016
DatePublished (current) - 4 Apr 2016
Issue number15
Volume55
Number of pages5
Pages (from-to)4738-4742
Early online date16/03/16
Original languageEnglish

Abstract

A highly efficient cap-exchange approach for preparing compact, dense polyvalent mannose-capped quantum dots (QDs) has been developed. The resulting QDs have been successfully used to probe multivalent interactions of HIV/Ebola receptors DC-SIGN and DC-SIGNR (collectively termed as DC-SIGN/R) using a sensitive, ratiometric Förster resonance energy transfer (FRET) assay. The QD probes specifically bind DC-SIGN, but not its closely related receptor DC-SIGNR, which is further confirmed by its specific blocking of DC-SIGN engagement with the Ebola virus glycoprotein. Tuning the QD surface mannose valency reveals that DC-SIGN binds more efficiently to densely packed mannosides. A FRET-based thermodynamic study reveals that the binding is enthalpy-driven. This work establishes QD FRET as a rapid, sensitive technique for probing structure and thermodynamics of multivalent protein-ligand interactions.

Bibliographical note

© 2016 The Authors.

    Research areas

  • FRET, carbohydrates, proteins, quantum dots, viral inhibition, Receptors, Cell Surface/chemistry, Molecular Probes/chemistry, Mannose/chemistry, Lectins, C-Type/chemistry, Proteins/chemistry, Cell Adhesion Molecules/chemistry, Quantum Dots, Fluorescence Resonance Energy Transfer, Ligands

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