Compartment-Specific Remodeling of Splenic Micro-Architecture during Experimental Visceral Leishmaniasis

Pinar Yurdakul, Jane Elise Dalton, Lynette Beattie, Nlajmeeyah Brown, Sibel Erguven, Asher Maroof, Paul M. Kaye

Research output: Contribution to journalArticlepeer-review


Progressive splenomegaly is a hallmark of visceral leishmaniasis in humans, canids, and rodents. In experimental murine visceral leishmaniasis, splenomegaly is accompanied by pronounced changes in microarchitecture, including expansion of the red pulp vascular system, neovascularization of the white pulp, and remodeling of the stromal cell populations that define the B-cell and T-cell compartments. Here, we show that Ly6C/G(+) (Gr-1(+)) cells, including neutrophils and inflammatory monocytes, accumulate in the splenic red pulp during infection. Cell depletion using monoclonal antibody against either Ly6C/G(+) (Gr-1; RB6) or Ly6G(+) (1A8) cells increased parasite burden. In contrast, depletion of Ly6C/G(+) cells, but not Ly6G(+) cells, halted the progressive remodeling of Meca-32(+) and CD31(+) red pulp vasculature. Strikingly, neither treatment affected white pulp neovascularization or the remodeling of the fibroblastic reticular cell and follicular dendritic cell networks. These findings demonstrate a previously unrecognized compartment-dependent selectivity to the process of splenic vascular remodeling during experimental murine visceral leishmaniasis, attributable to Ly6C(+) inflammatory monocytes. (Am J Palbol 2011, 179:23-29; 1)01: 10.1016/j.ajpath.2011.03.009)

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalAmerican Journal of Pathology
Issue number1
Publication statusPublished - Jul 2011

Bibliographical note

Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.


  • Animals
  • Antibodies, Monoclonal
  • Antigens, Ly
  • B-Lymphocytes
  • Blotting, Western
  • Cells, Cultured
  • Cricetinae
  • Dendritic Cells
  • Female
  • Fibroblasts
  • Flow Cytometry
  • Immunoenzyme Techniques
  • Leishmania donovani
  • Leishmaniasis, Visceral
  • Macrophages
  • Mesocricetus
  • Mice
  • Mice, Inbred C57BL
  • Monocytes
  • Neovascularization, Pathologic
  • Neutrophils
  • Spleen
  • Splenomegaly

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