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Compromised Virus-Induced Gene Silencing in RDR6-Deficient Plants

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JournalPlant Physiology
DatePublished - Mar 2009
Issue number3
Volume149
Number of pages9
Pages (from-to)1399-1407
Original languageEnglish

Abstract

RNA silencing in plants serves as a potent antiviral defense mechanism through the action of small interfering RNAs (siRNAs), which direct RNA degradation. siRNAs can be derived directly from the viral genome or via the action of host-encoded RNA-dependent RNA polymerases (RDRs). Plant genomes encode multiple RDRs, and it has been demonstrated that plants defective for RDR6 hyperaccumulate several classes of virus. In this study, we compared the effectiveness of virus-induced gene silencing (VIGS) and RNA-directed DNA methylation (RdDM) in wild-type and RDR6-deficient Nicotiana benthamiana plants. For the potexvirus Potato virus X (PVX) and the potyvirus Plum pox virus (PPV), the efficiency of both VIGS and RdDM were compromised in RDR6-defective plants despite accumulating high levels of viral siRNAs similar to infection of wild-type plants. The reduced efficiency of VIGS and RdDM was unrelated to the size class of siRNA produced and, at least for PVX, was not dependent on the presence of the virus-encoded silencing suppressor protein, 25K. We suggest that primary siRNAs produced from PVX and PPV in the absence of RDR6 may not be good effectors of silencing and that RDR6 is required to produce secondary siRNAs that drive a more effective antiviral response.

    Research areas

  • DEPENDENT RNA-POLYMERASE, SMALL INTERFERING RNAS, TRANS-ACTING SIRNAS, DOUBLE-STRANDED-RNA, TO-CELL MOVEMENT, NICOTIANA-BENTHAMIANA, ARABIDOPSIS-THALIANA, ANTIVIRAL DEFENSE, DNA METHYLATION, TARGET GENE

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