Abstract
An oligonucleotide, encoding a short epitope peptide tag, termed Pk, was inserted at the 3'-end of the gene coding B-subunit of Escherichia coli heat-labile enterotoxin (LTB). The presence of the Pk epitope on LTB-Pk was used to construct novel macromolecular assemblies comprising LTB-Pk, an anti-Pk mAb, (mAb SV5-P-k) and Pk-linked recombinant SIV proteins. The 1:1:1 stoichiometry of such complexes was ensured by binding LTB-Pk to one arm of mAb SV5-P-k and an SIV-Pk antigen to the other arm of the antibody. Such SIV-mAb-LTB macromolecular complexes bound to GM1-ganglioside in vitro, and when immunized systemically into mice were highly immunogenic, inducing both humoral and cell-mediated responses to the recombinant SIV antigens.
Original language | English |
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Pages (from-to) | 949-58 |
Number of pages | 10 |
Journal | Vaccine |
Volume | 14 |
Issue number | 10 |
Publication status | Published - 1996 |
Keywords
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Formation
- Antigen-Antibody Reactions
- Bacterial Toxins
- Base Sequence
- Binding Sites, Antibody
- Enterotoxins
- Escherichia coli Proteins
- G(M1) Ganglioside
- Immunity, Cellular
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Simian immunodeficiency virus
- Vaccines, Synthetic