Contrast-tuneable microscopy for single-shot real-time imaging

Isao Shimizu, Yoshinori Saikawa, Katsunori Uno, Hideaki Kano, Seishi Shimizu

Research output: Contribution to journalArticlepeer-review


A novel real image in-line laser holography enables a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered light. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.
Original languageEnglish
Article number30701
Number of pages8
JournalThe European Physical Journal Applied Physics
Issue number3
Publication statusPublished - 18 Sept 2020

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