Contrast-tuneable microscopy for single-shot real-time imaging

Isao Shimizu; Yoshinori Saikawa; Katsunori Uno; Hideaki Kano; Seishi Shimizu

doi:10.1051/epjap/2020200101

Contrast-tuneable microscopy for single-shot real-time imaging

Isao Shimizu, Yoshinori Saikawa, Katsunori Uno, Hideaki Kano, Seishi Shimizu

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A novel real image in-line laser holography enables a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered light. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.

Original language	English
Article number	30701
Number of pages	8
Journal	The European Physical Journal Applied Physics
Volume	91
Issue number	3
DOIs	https://doi.org/10.1051/epjap/2020200101
Publication status	Published - 18 Sept 2020

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10.1051/epjap/2020200101

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Cite this

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title = "Contrast-tuneable microscopy for single-shot real-time imaging",

abstract = "A novel real image in-line laser holography enables a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered light. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics. ",

author = "Isao Shimizu and Yoshinori Saikawa and Katsunori Uno and Hideaki Kano and Seishi Shimizu",

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AU - Shimizu, Isao

AU - Saikawa, Yoshinori

AU - Uno, Katsunori

AU - Kano, Hideaki

AU - Shimizu, Seishi

PY - 2020/9/18

Y1 - 2020/9/18

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AB - A novel real image in-line laser holography enables a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered light. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.

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DO - 10.1051/epjap/2020200101

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