Control of germination and lipid mobilization by COMATOSE, the Arabidopsis homologue of human ALDP

S Footitt; S P Slocombe; V Larner; S Kurup; Y S Wu; T Larson; I Graham; A Baker; M Holdsworth

Control of germination and lipid mobilization by COMATOSE, the Arabidopsis homologue of human ALDP

S Footitt, S P Slocombe, V Larner, S Kurup, Y S Wu, T Larson, I Graham, A Baker, M Holdsworth

CNAP

Research output: Contribution to journal › Article › peer-review

Abstract

Embryo dormancy in flowering plants is an important dispersal mechanism that promotes survival of the seed through time. The subsequent transition to germination is a critical control point regulating initiation of vegetative growth. Here we show that the Arabidopsis COMATOSE (CTS) locus is required for this transition, and acts, at least in part, by profoundly affecting the metabolism of stored lipids. CTS encodes a peroxisomal protein of the ATP binding cassette (ABC) transporter class with significant identity to the human X-linked adrenoleukodystrophy protein (ALDP). Like X-ALD patients, cts mutant embryos and seedlings exhibit pleiotropic phenotypes associated with perturbation in fatty acid metabolism. CTS expression transiently increases shortly after imbibition during germination, but not in imbibed dormant seeds, and genetic analyses show that CTS is negatively regulated by loci that promote embryo dormancy through multiple independent pathways. Our results demonstrate that CTS regulates transport of acyl CoAs into the peroxisome, and indicate that regulation of CTS function is a major control point for the switch between the opposing developmental programmes of dormancy and germination.

Original language	English
Pages (from-to)	2912-2922
Number of pages	11
Journal	EMBO Journal
Volume	21
Issue number	12
Publication status	Published - 17 Jun 2002

Keywords

ABC lipid transporter
adrenoleukodystrophy
Arabidopsis
dormancy
germination
ACID BETA-OXIDATION
BINDING-CASSETTE TRANSPORTER
X-LINKED ADRENOLEUKODYSTROPHY
PEROXISOMAL MEMBRANE-PROTEIN
ACYL-COA SYNTHETASE
EMBRYO DEVELOPMENT
SACCHAROMYCES-CEREVISIAE
ABC TRANSPORTERS
LEAFY COTYLEDON1
FATTY-ACIDS

Cite this

@article{6472b30bdb884abc8d81edc1e2aed0d4,

title = "Control of germination and lipid mobilization by COMATOSE, the Arabidopsis homologue of human ALDP",

abstract = "Embryo dormancy in flowering plants is an important dispersal mechanism that promotes survival of the seed through time. The subsequent transition to germination is a critical control point regulating initiation of vegetative growth. Here we show that the Arabidopsis COMATOSE (CTS) locus is required for this transition, and acts, at least in part, by profoundly affecting the metabolism of stored lipids. CTS encodes a peroxisomal protein of the ATP binding cassette (ABC) transporter class with significant identity to the human X-linked adrenoleukodystrophy protein (ALDP). Like X-ALD patients, cts mutant embryos and seedlings exhibit pleiotropic phenotypes associated with perturbation in fatty acid metabolism. CTS expression transiently increases shortly after imbibition during germination, but not in imbibed dormant seeds, and genetic analyses show that CTS is negatively regulated by loci that promote embryo dormancy through multiple independent pathways. Our results demonstrate that CTS regulates transport of acyl CoAs into the peroxisome, and indicate that regulation of CTS function is a major control point for the switch between the opposing developmental programmes of dormancy and germination.",

keywords = "ABC lipid transporter, adrenoleukodystrophy, Arabidopsis, dormancy, germination, ACID BETA-OXIDATION, BINDING-CASSETTE TRANSPORTER, X-LINKED ADRENOLEUKODYSTROPHY, PEROXISOMAL MEMBRANE-PROTEIN, ACYL-COA SYNTHETASE, EMBRYO DEVELOPMENT, SACCHAROMYCES-CEREVISIAE, ABC TRANSPORTERS, LEAFY COTYLEDON1, FATTY-ACIDS",

author = "S Footitt and Slocombe, {S P} and V Larner and S Kurup and Wu, {Y S} and T Larson and I Graham and A Baker and M Holdsworth",

year = "2002",

month = jun,

day = "17",

language = "English",

volume = "21",

pages = "2912--2922",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Springer",

number = "12",

}

TY - JOUR

T1 - Control of germination and lipid mobilization by COMATOSE, the Arabidopsis homologue of human ALDP

AU - Footitt, S

AU - Slocombe, S P

AU - Larner, V

AU - Kurup, S

AU - Wu, Y S

AU - Larson, T

AU - Graham, I

AU - Baker, A

AU - Holdsworth, M

PY - 2002/6/17

Y1 - 2002/6/17

N2 - Embryo dormancy in flowering plants is an important dispersal mechanism that promotes survival of the seed through time. The subsequent transition to germination is a critical control point regulating initiation of vegetative growth. Here we show that the Arabidopsis COMATOSE (CTS) locus is required for this transition, and acts, at least in part, by profoundly affecting the metabolism of stored lipids. CTS encodes a peroxisomal protein of the ATP binding cassette (ABC) transporter class with significant identity to the human X-linked adrenoleukodystrophy protein (ALDP). Like X-ALD patients, cts mutant embryos and seedlings exhibit pleiotropic phenotypes associated with perturbation in fatty acid metabolism. CTS expression transiently increases shortly after imbibition during germination, but not in imbibed dormant seeds, and genetic analyses show that CTS is negatively regulated by loci that promote embryo dormancy through multiple independent pathways. Our results demonstrate that CTS regulates transport of acyl CoAs into the peroxisome, and indicate that regulation of CTS function is a major control point for the switch between the opposing developmental programmes of dormancy and germination.

AB - Embryo dormancy in flowering plants is an important dispersal mechanism that promotes survival of the seed through time. The subsequent transition to germination is a critical control point regulating initiation of vegetative growth. Here we show that the Arabidopsis COMATOSE (CTS) locus is required for this transition, and acts, at least in part, by profoundly affecting the metabolism of stored lipids. CTS encodes a peroxisomal protein of the ATP binding cassette (ABC) transporter class with significant identity to the human X-linked adrenoleukodystrophy protein (ALDP). Like X-ALD patients, cts mutant embryos and seedlings exhibit pleiotropic phenotypes associated with perturbation in fatty acid metabolism. CTS expression transiently increases shortly after imbibition during germination, but not in imbibed dormant seeds, and genetic analyses show that CTS is negatively regulated by loci that promote embryo dormancy through multiple independent pathways. Our results demonstrate that CTS regulates transport of acyl CoAs into the peroxisome, and indicate that regulation of CTS function is a major control point for the switch between the opposing developmental programmes of dormancy and germination.

KW - ABC lipid transporter

KW - adrenoleukodystrophy

KW - Arabidopsis

KW - dormancy

KW - germination

KW - ACID BETA-OXIDATION

KW - BINDING-CASSETTE TRANSPORTER

KW - X-LINKED ADRENOLEUKODYSTROPHY

KW - PEROXISOMAL MEMBRANE-PROTEIN

KW - ACYL-COA SYNTHETASE

KW - EMBRYO DEVELOPMENT

KW - SACCHAROMYCES-CEREVISIAE

KW - ABC TRANSPORTERS

KW - LEAFY COTYLEDON1

KW - FATTY-ACIDS

M3 - Article

SN - 0261-4189

VL - 21

SP - 2912

EP - 2922

JO - EMBO Journal

JF - EMBO Journal

IS - 12

ER -