Control of recruitment and transcription-activating function of CBP determines gene regulation by NMDA receptors and L-type calcium channels

G E Hardingham, S Chawla, F H Cruzalegui, H Bading

Research output: Contribution to journalArticlepeer-review


Recruitment of the coactivator CBP by signal-regulated transcription factors and stimulation of CBP activity are key regulatory events in the induction of gene transcription following Ca2+ flux through ligand- and/or voltage-gated ion channels in hippocampal neurons. The mode of Ca2+ entry (L-type Ca2+ channels versus NMDA receptors) differentially controls the CBP recruitment step to CREB, providing a molecular basis for the observed Ca2+ channel type-dependent differences in gene expression. In contrast, activation of CBP is triggered irrespective of the route of Ca2+ entry, as is activation of c-Jun, that recruits CBP independently of phosphorylation at major regulatory c-Jun phosphorylation sites, serines 63 and 73. This control of CBP recruitment and activation is likely relevant to other CBP-interacting transcription factors and represents a general mechanism through which Ca2+ signals associated with electrical activity may regulate the expression of many genes.
Original languageEnglish
Pages (from-to)789-98
Number of pages10
Issue number4
Publication statusPublished - 1999


  • CREB-Binding Protein
  • Calcium
  • Calcium Channels
  • Cells, Cultured
  • Gene Expression Regulation
  • Hippocampus
  • Humans
  • Membrane Potentials
  • Nerve Tissue Proteins
  • Neurons
  • Nuclear Proteins
  • Phosphorylation
  • Proto-Oncogene Proteins c-fos
  • Receptors, N-Methyl-D-Aspartate
  • Recruitment, Neurophysiological
  • Trans-Activators
  • Transcriptional Activation

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