Controlled Release of DNA From Photoresponsive Hyperbranched Polyglycerols with Oligoamine Shells

Wiebke Fischer; Mohiuddin A. Quadir; Anna Barnard; David K. Smith; Rainer Haag

doi:10.1002/mabi.201100248

Controlled Release of DNA From Photoresponsive Hyperbranched Polyglycerols with Oligoamine Shells

Wiebke Fischer, Mohiuddin A. Quadir, Anna Barnard, David K. Smith, Rainer Haag

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Two photo-responsive core/shell nanoparticles based on hyperbranched polyglycerol (hPG) are synthesized for controlled release of DNA. The shell is composed either of bis-(3-aminopropyl)methylamine (AMPA) or pentaethylenehexamine (PEHA) derivatives and is attached to the hPG core with a photo-responsive o-nitrobenzyl linker. Ethidium bromide displacement assay, gel electrophoresis, DLS, and ?-potential measurements are performed with these nanoparticles. Photo-responsive changes within the carrier scaffold are investigated by irradiating the polymer solution with 350?nm monochromatic light. Fully covered APMA-shelled carriers are found to complex the DNA at an N/P ratio of 10 with an average size ranging from 54 to 78?nm depending on the degree of functionalization of the core.

Original language	English
Pages (from-to)	1736-1746
Number of pages	11
Journal	Macromolecular bioscience
Volume	11
Issue number	12
DOIs	https://doi.org/10.1002/mabi.201100248
Publication status	Published - 8 Dec 2011

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abstract = "Two photo-responsive core/shell nanoparticles based on hyperbranched polyglycerol (hPG) are synthesized for controlled release of DNA. The shell is composed either of bis-(3-aminopropyl)methylamine (AMPA) or pentaethylenehexamine (PEHA) derivatives and is attached to the hPG core with a photo-responsive o-nitrobenzyl linker. Ethidium bromide displacement assay, gel electrophoresis, DLS, and ?-potential measurements are performed with these nanoparticles. Photo-responsive changes within the carrier scaffold are investigated by irradiating the polymer solution with 350?nm monochromatic light. Fully covered APMA-shelled carriers are found to complex the DNA at an N/P ratio of 10 with an average size ranging from 54 to 78?nm depending on the degree of functionalization of the core.",

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AU - Haag, Rainer

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Controlled Release of DNA From Photoresponsive Hyperbranched Polyglycerols with Oligoamine Shells

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