Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells

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Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.

Original languageEnglish
Pages (from-to)183-190
Number of pages8
JournalMethods in Molecular Biology
Publication statusPublished - 31 May 2022

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  • Carrier Proteins/metabolism
  • DNA Copy Number Variations
  • Histone Deacetylases/metabolism
  • Proteins/metabolism
  • Saccharomyces cerevisiae/genetics
  • Saccharomyces cerevisiae Proteins/genetics

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