Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells

Sviatlana Shashkova; Thomas Nyström; Mark C Leake

doi:10.1007/978-1-0716-2221-6_14

Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells

Sviatlana Shashkova, Thomas Nyström, Mark C Leake

Research output: Contribution to journal › Article › peer-review

Abstract

Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.

Original language	English
Pages (from-to)	183-190
Number of pages	8
Journal	Methods in Molecular Biology
Volume	2476
DOIs	https://doi.org/10.1007/978-1-0716-2221-6_14
Publication status	Published - 31 May 2022

Bibliographical note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

Keywords

Carrier Proteins/metabolism
DNA Copy Number Variations
Histone Deacetylases/metabolism
Proteins/metabolism
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae Proteins/genetics

Access to Document

10.1007/978-1-0716-2221-6_14

text figures Shashkova
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details
Accepted author manuscript, 581 KB

Cite this

@article{34ceaacbf6854084958223a85726cfd8,

title = "Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells",

abstract = "Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.",

keywords = "Carrier Proteins/metabolism, DNA Copy Number Variations, Histone Deacetylases/metabolism, Proteins/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics",

author = "Sviatlana Shashkova and Thomas Nystr{\"o}m and Leake, {Mark C}",

note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This is an author-produced version of the published paper. Uploaded in accordance with the publisher{\textquoteright}s self-archiving policy. Further copying may not be permitted; contact the publisher for details",

year = "2022",

month = may,

day = "31",

doi = "10.1007/978-1-0716-2221-6_14",

language = "English",

volume = "2476",

pages = "183--190",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells

AU - Shashkova, Sviatlana

AU - Nyström, Thomas

AU - Leake, Mark C

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

PY - 2022/5/31

Y1 - 2022/5/31

N2 - Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.

AB - Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.

KW - Carrier Proteins/metabolism

KW - DNA Copy Number Variations

KW - Histone Deacetylases/metabolism

KW - Proteins/metabolism

KW - Saccharomyces cerevisiae/genetics

KW - Saccharomyces cerevisiae Proteins/genetics

U2 - 10.1007/978-1-0716-2221-6_14

DO - 10.1007/978-1-0716-2221-6_14

M3 - Article

C2 - 35635705

SN - 1064-3745

VL - 2476

SP - 183

EP - 190

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -