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From the same journal

Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells

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Publication details

JournalMethods in Molecular Biology
DateAccepted/In press - 14 Feb 2022
DatePublished (current) - 31 May 2022
Volume2476
Number of pages8
Pages (from-to)183-190
Original languageEnglish

Abstract

Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.

Bibliographical note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

    Research areas

  • Carrier Proteins/metabolism, DNA Copy Number Variations, Histone Deacetylases/metabolism, Proteins/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics

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