CRISPR/Cas9 gene editing in the West Nile Virus vector, Culex quinquefasciatus Say

Michelle E. Anderson; Jessica Mavica; Lewis Shackleford; Ilona Flis; Sophia Fochler; Sanjay Basu; Luke Alphey

doi:10.1371/journal.pone.0224857

CRISPR/Cas9 gene editing in the West Nile Virus vector, Culex quinquefasciatus Say

Michelle E. Anderson, Jessica Mavica, Lewis Shackleford, Ilona Flis, Sophia Fochler, Sanjay Basu, Luke Alphey^*

^*Corresponding author for this work

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Culex quinquefasciatus Say is an opportunistic blood feeder with a wide geographic distribution which is also a major vector for a range of diseases of both animals and humans. CRISPR/Cas technologies have been applied to a wide variety of organisms for both applied and basic research purposes. CRISPR/Cas methods open new possibilities for genetic research in non-model organisms of public health importance. In this work we have adapted microinjection techniques commonly used in other mosquito species to Culex quinquefasciatus, and have shown these to be effective at generating homozygous knock-out mutations of a target gene in one generation. This is the first description of the kmo gene and mutant phenotype in this species.

Original language	English
Article number	e0224857
Journal	PLOS ONE
Volume	14
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0224857
Publication status	Published - 1 Nov 2019

Bibliographical note

Publisher Copyright:
© 2019 Anderson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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AU - Fochler, Sophia

AU - Basu, Sanjay

AU - Alphey, Luke

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CRISPR/Cas9 gene editing in the West Nile Virus vector, Culex quinquefasciatus Say

Abstract

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