Crustacean cardioactive peptide (CCAP)-related molluscan peptides (M-CCAPs) are potential extrinsic modulators of the buccal feeding network in the pond snail Lymnaea stagnalis

A Vehovszky; H J Agricola; C J H Elliott; M Ohtani; L Karpati; L Hernadi

doi:10.1016/j.neulet.2004.10.020

Crustacean cardioactive peptide (CCAP)-related molluscan peptides (M-CCAPs) are potential extrinsic modulators of the buccal feeding network in the pond snail Lymnaea stagnalis

A Vehovszky, H J Agricola, C J H Elliott, M Ohtani, L Karpati, L Hernadi

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We combine electrophysiological and immunocytochemical analyses in the snail Lymnaea stagnalis of M-CCAP1 and M-CCAP2, two molluscan peptides with structure similar to crustacean cardioactive peptide CCAP, originally isolated from the snail Helix pomatia. Both M-CCAP peptides (M-CCAP1 and M-CCAP2, 1 muM) had an excitatory effect, depolarizing all the identified neurons of the buccal feeding network (including motoneurons: B 1, B2, B4 and modulatory interneurons SO, OC: 62 neurons in 33 preparations). Additionally, in 67% of preparations, rhythmic activity (fictive feeding) was recorded with a mean rate of 7 cycles/min. No significant difference in the proportion of. preparations showing fictive feeding or mean feeding rate was found between M-CCAP1 and M-CCAP2. The extrinsic feeding modulator, the serotonergic CGC neuron, responds by increase of the spontaneous activity after M-CCAP application (9 of 18 preparations). Crustacean CCAP (1 muM) evokes a slight membrane depolarization in 3 out of 8 preparations but never evokes fictive feeding.

Immunostaining revealed no cell bodies in the buccal ganglia, but a dense network of CCAP immunopositive fibers arborizing in the buccal neuropil. Many of these fibers originate from a symmetrical pair of CCAP-immunoreactive cerebro-buccal interneurons, which are the most likely candidates for extrinsic modulatory interneurons in the buccal feeding network. Our data are the first results suggesting that M-CCAP-peptides exist as effective modulators in mollusc. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

Original language	English
Pages (from-to)	200-205
Number of pages	6
Journal	NEUROSCIENCE LETTERS
Volume	373
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2004.10.020
Publication status	Published - 20 Jan 2005

Keywords

central pattern generator
CPG
feeding
neuromodulation
mollusc
electrophysiology
immunocytochemistry
CRAB CARCINUS-MAENAS
SHORE CRAB
NERVOUS-SYSTEM
NEURONS
INTERNEURONS
ACTIVATION
DROSOPHILA
BEHAVIOR
ECDYSIS

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10.1016/j.neulet.2004.10.020

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@article{45c6010500dc4c73b3275ce5eb700866,

title = "Crustacean cardioactive peptide (CCAP)-related molluscan peptides (M-CCAPs) are potential extrinsic modulators of the buccal feeding network in the pond snail Lymnaea stagnalis",

abstract = "We combine electrophysiological and immunocytochemical analyses in the snail Lymnaea stagnalis of M-CCAP1 and M-CCAP2, two molluscan peptides with structure similar to crustacean cardioactive peptide CCAP, originally isolated from the snail Helix pomatia. Both M-CCAP peptides (M-CCAP1 and M-CCAP2, 1 muM) had an excitatory effect, depolarizing all the identified neurons of the buccal feeding network (including motoneurons: B 1, B2, B4 and modulatory interneurons SO, OC: 62 neurons in 33 preparations). Additionally, in 67% of preparations, rhythmic activity (fictive feeding) was recorded with a mean rate of 7 cycles/min. No significant difference in the proportion of. preparations showing fictive feeding or mean feeding rate was found between M-CCAP1 and M-CCAP2. The extrinsic feeding modulator, the serotonergic CGC neuron, responds by increase of the spontaneous activity after M-CCAP application (9 of 18 preparations). Crustacean CCAP (1 muM) evokes a slight membrane depolarization in 3 out of 8 preparations but never evokes fictive feeding.Immunostaining revealed no cell bodies in the buccal ganglia, but a dense network of CCAP immunopositive fibers arborizing in the buccal neuropil. Many of these fibers originate from a symmetrical pair of CCAP-immunoreactive cerebro-buccal interneurons, which are the most likely candidates for extrinsic modulatory interneurons in the buccal feeding network. Our data are the first results suggesting that M-CCAP-peptides exist as effective modulators in mollusc. (C) 2004 Elsevier Ireland Ltd. All rights reserved.",

keywords = "central pattern generator, CPG, feeding, neuromodulation, mollusc, electrophysiology, immunocytochemistry, CRAB CARCINUS-MAENAS, SHORE CRAB, NERVOUS-SYSTEM, NEURONS, INTERNEURONS, ACTIVATION, DROSOPHILA, BEHAVIOR, ECDYSIS",

author = "A Vehovszky and Agricola, {H J} and Elliott, {C J H} and M Ohtani and L Karpati and L Hernadi",

year = "2005",

month = jan,

day = "20",

doi = "10.1016/j.neulet.2004.10.020",

language = "English",

volume = "373",

pages = "200--205",

journal = "NEUROSCIENCE LETTERS",

issn = "0304-3940",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Crustacean cardioactive peptide (CCAP)-related molluscan peptides (M-CCAPs) are potential extrinsic modulators of the buccal feeding network in the pond snail Lymnaea stagnalis

AU - Vehovszky, A

AU - Agricola, H J

AU - Elliott, C J H

AU - Ohtani, M

AU - Karpati, L

AU - Hernadi, L

PY - 2005/1/20

Y1 - 2005/1/20

N2 - We combine electrophysiological and immunocytochemical analyses in the snail Lymnaea stagnalis of M-CCAP1 and M-CCAP2, two molluscan peptides with structure similar to crustacean cardioactive peptide CCAP, originally isolated from the snail Helix pomatia. Both M-CCAP peptides (M-CCAP1 and M-CCAP2, 1 muM) had an excitatory effect, depolarizing all the identified neurons of the buccal feeding network (including motoneurons: B 1, B2, B4 and modulatory interneurons SO, OC: 62 neurons in 33 preparations). Additionally, in 67% of preparations, rhythmic activity (fictive feeding) was recorded with a mean rate of 7 cycles/min. No significant difference in the proportion of. preparations showing fictive feeding or mean feeding rate was found between M-CCAP1 and M-CCAP2. The extrinsic feeding modulator, the serotonergic CGC neuron, responds by increase of the spontaneous activity after M-CCAP application (9 of 18 preparations). Crustacean CCAP (1 muM) evokes a slight membrane depolarization in 3 out of 8 preparations but never evokes fictive feeding.Immunostaining revealed no cell bodies in the buccal ganglia, but a dense network of CCAP immunopositive fibers arborizing in the buccal neuropil. Many of these fibers originate from a symmetrical pair of CCAP-immunoreactive cerebro-buccal interneurons, which are the most likely candidates for extrinsic modulatory interneurons in the buccal feeding network. Our data are the first results suggesting that M-CCAP-peptides exist as effective modulators in mollusc. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

AB - We combine electrophysiological and immunocytochemical analyses in the snail Lymnaea stagnalis of M-CCAP1 and M-CCAP2, two molluscan peptides with structure similar to crustacean cardioactive peptide CCAP, originally isolated from the snail Helix pomatia. Both M-CCAP peptides (M-CCAP1 and M-CCAP2, 1 muM) had an excitatory effect, depolarizing all the identified neurons of the buccal feeding network (including motoneurons: B 1, B2, B4 and modulatory interneurons SO, OC: 62 neurons in 33 preparations). Additionally, in 67% of preparations, rhythmic activity (fictive feeding) was recorded with a mean rate of 7 cycles/min. No significant difference in the proportion of. preparations showing fictive feeding or mean feeding rate was found between M-CCAP1 and M-CCAP2. The extrinsic feeding modulator, the serotonergic CGC neuron, responds by increase of the spontaneous activity after M-CCAP application (9 of 18 preparations). Crustacean CCAP (1 muM) evokes a slight membrane depolarization in 3 out of 8 preparations but never evokes fictive feeding.Immunostaining revealed no cell bodies in the buccal ganglia, but a dense network of CCAP immunopositive fibers arborizing in the buccal neuropil. Many of these fibers originate from a symmetrical pair of CCAP-immunoreactive cerebro-buccal interneurons, which are the most likely candidates for extrinsic modulatory interneurons in the buccal feeding network. Our data are the first results suggesting that M-CCAP-peptides exist as effective modulators in mollusc. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

KW - central pattern generator

KW - CPG

KW - feeding

KW - neuromodulation

KW - mollusc

KW - electrophysiology

KW - immunocytochemistry

KW - CRAB CARCINUS-MAENAS

KW - SHORE CRAB

KW - NERVOUS-SYSTEM

KW - NEURONS

KW - INTERNEURONS

KW - ACTIVATION

KW - DROSOPHILA

KW - BEHAVIOR

KW - ECDYSIS

U2 - 10.1016/j.neulet.2004.10.020

DO - 10.1016/j.neulet.2004.10.020

M3 - Article

SN - 0304-3940

VL - 373

SP - 200

EP - 205

JO - NEUROSCIENCE LETTERS

JF - NEUROSCIENCE LETTERS

IS - 3

ER -