By the same authors

From the same journal

Crystallization and preliminary crystallographic analysis of deoxyuridine 5 '-triphosphate nucleotidohydrolase from Bacillus subtilis

Research output: Contribution to journalArticle

Author(s)

Department/unit(s)

Publication details

JournalActa Crystallographica Section D: Biological Crystallography
DatePublished - Jun 2001
Volume57
Number of pages3
Pages (from-to)876-878
Original languageEnglish

Abstract

Single crystals of purified homotrimeric deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) from Bacillus subtilis have been grown under several different conditions using vapour diffusion. X-ray diffraction data have been collected using synchrotron radiation from three crystal forms of the unliganded enzyme and from enzyme cocrystallized with a substrate analogue and inhibitor, dUDP, and a metal ion, Sr2+. The three crystal forms of unliganded enzyme belong to hexagonal (P6(3)), orthorhombic (P2(1)2(1)2) and cubic (P2(1)3) space groups and data have been recorded to 1.75, 1.90 and 2.50 Angstrom spacing, respectively. Crystals grown in the presence of dUDP and Sr2+ belong to the orthorhombic space group P2(1)2(1)2(1) and data were measured to 1.90 Angstrom spacing. Solution of the hexagonal crystal form by molecular replacement using the dUTPase from feline immunodeficiency virus as a search model is in progress.

    Research areas

  • FELINE IMMUNODEFICIENCY VIRUS, INFECTIOUS-ANEMIA VIRUS, CRYSTAL-STRUCTURE, DUTP PYROPHOSPHATASE, ESCHERICHIA-COLI, COMPLEXES, DNA

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations