Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus

V  Ducros; G J  Davies; D M  Lawson; K S  Wilson; S H  Brown; P  Ostergaard; A H  Pedersen; P  Schneider; D S  Yaver; A M  Brzozowski

Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus

V Ducros, G J Davies, D M Lawson, K S Wilson, S H Brown, P Ostergaard, A H Pedersen, P Schneider, D S Yaver, A M Brzozowski

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The laccase from the fungus Coprinus cinereus has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small plate-like crystals of an enzymatically deglycosylated form of the enzyme have been grown by the hanging-drop method using polyethylene glycol as precipitant. These crystals diffract to at least 2.2 Angstrom. They belong to the space group P2(1)2(1)2(1), with cell dimensions a = 45.4, b = 85.7, c = 143.1 Angstrom with a single molecule of laccase in the asymmetric unit.

Original language	English
Pages (from-to)	605-607
Number of pages	3
Journal	Acta Crystallographica. Section D, Biological Crystallography
Volume	53
Publication status	Published - 1 Sept 1997

Keywords

CRYSTAL-STRUCTURE
ASCORBATE OXIDASE
RESOLUTION
EXPRESSION
OXIDATION
GENES

Cite this

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title = "Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus",

abstract = "The laccase from the fungus Coprinus cinereus has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small plate-like crystals of an enzymatically deglycosylated form of the enzyme have been grown by the hanging-drop method using polyethylene glycol as precipitant. These crystals diffract to at least 2.2 Angstrom. They belong to the space group P2(1)2(1)2(1), with cell dimensions a = 45.4, b = 85.7, c = 143.1 Angstrom with a single molecule of laccase in the asymmetric unit.",

keywords = "CRYSTAL-STRUCTURE, ASCORBATE OXIDASE, RESOLUTION, EXPRESSION, OXIDATION, GENES",

author = "V Ducros and Davies, {G J} and Lawson, {D M} and Wilson, {K S} and Brown, {S H} and P Ostergaard and Pedersen, {A H} and P Schneider and Yaver, {D S} and Brzozowski, {A M}",

year = "1997",

month = sep,

day = "1",

language = "English",

volume = "53",

pages = "605--607",

journal = "Acta Crystallographica. Section D, Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

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TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus

AU - Ducros, V

AU - Davies, G J

AU - Lawson, D M

AU - Wilson, K S

AU - Brown, S H

AU - Ostergaard, P

AU - Pedersen, A H

AU - Schneider, P

AU - Yaver, D S

AU - Brzozowski, A M

PY - 1997/9/1

Y1 - 1997/9/1

N2 - The laccase from the fungus Coprinus cinereus has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small plate-like crystals of an enzymatically deglycosylated form of the enzyme have been grown by the hanging-drop method using polyethylene glycol as precipitant. These crystals diffract to at least 2.2 Angstrom. They belong to the space group P2(1)2(1)2(1), with cell dimensions a = 45.4, b = 85.7, c = 143.1 Angstrom with a single molecule of laccase in the asymmetric unit.

AB - The laccase from the fungus Coprinus cinereus has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small plate-like crystals of an enzymatically deglycosylated form of the enzyme have been grown by the hanging-drop method using polyethylene glycol as precipitant. These crystals diffract to at least 2.2 Angstrom. They belong to the space group P2(1)2(1)2(1), with cell dimensions a = 45.4, b = 85.7, c = 143.1 Angstrom with a single molecule of laccase in the asymmetric unit.

KW - CRYSTAL-STRUCTURE

KW - ASCORBATE OXIDASE

KW - RESOLUTION

KW - EXPRESSION

KW - OXIDATION

KW - GENES

M3 - Article

SN - 0907-4449

VL - 53

SP - 605

EP - 607

JO - Acta Crystallographica. Section D, Biological Crystallography

JF - Acta Crystallographica. Section D, Biological Crystallography

ER -