Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5

E  Sabini; A M  Brzozowski; M  Dauter; G J  Davies; K S  Wilson; M  Paloheimo; P  Suominen; M  Siika-Aho; M  Penttila

Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5

E Sabini, A M Brzozowski, M Dauter, G J Davies, K S Wilson, M Paloheimo, P Suominen, M Siika-Aho, M Penttila

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Crystals of the catalytic core domain of a Trichoderma reesei beta-mannanase belonging to glycoside hydrolase family 5 have been grown by the sitting-drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P2(1), with unit-cell parameters a = 50.0, b = 54.3, c = 60.2 Angstrom, beta = 111.3 degrees, and have a single monomer of mannanase in the asymmetric unit. Native data to 2.0 Angstrom resolution have been collected at room temperature using synchrotron radiation. Data for a platinum derivative have been collected to 1.65 Angstrom at 110 K in a very short time at the CCLRC Daresbury synchrotron source, using a charge-coupled device (CCD) as detector.

Original language	English
Pages (from-to)	1058-1060
Number of pages	3
Journal	Acta Crystallographica. Section D, Biological Crystallography
Volume	55
Publication status	Published - May 1999

Keywords

SEQUENCE-BASED CLASSIFICATION
SACCHAROMYCES-CEREVISIAE
CRYSTAL-STRUCTURE
ENDOGLUCANASE
CELLULASE
COMPLEX
DOMAIN
GENE

Cite this

@article{07f399407f7a47e9ace5a08651a808a8,

title = "Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5",

abstract = "Crystals of the catalytic core domain of a Trichoderma reesei beta-mannanase belonging to glycoside hydrolase family 5 have been grown by the sitting-drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P2(1), with unit-cell parameters a = 50.0, b = 54.3, c = 60.2 Angstrom, beta = 111.3 degrees, and have a single monomer of mannanase in the asymmetric unit. Native data to 2.0 Angstrom resolution have been collected at room temperature using synchrotron radiation. Data for a platinum derivative have been collected to 1.65 Angstrom at 110 K in a very short time at the CCLRC Daresbury synchrotron source, using a charge-coupled device (CCD) as detector.",

keywords = "SEQUENCE-BASED CLASSIFICATION, SACCHAROMYCES-CEREVISIAE, CRYSTAL-STRUCTURE, ENDOGLUCANASE, CELLULASE, COMPLEX, DOMAIN, GENE",

author = "E Sabini and Brzozowski, {A M} and M Dauter and Davies, {G J} and Wilson, {K S} and M Paloheimo and P Suominen and M Siika-Aho and M Penttila",

year = "1999",

month = may,

language = "English",

volume = "55",

pages = "1058--1060",

journal = "Acta Crystallographica. Section D, Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

}

TY - JOUR

T1 - Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5

AU - Sabini, E

AU - Brzozowski, A M

AU - Dauter, M

AU - Davies, G J

AU - Wilson, K S

AU - Paloheimo, M

AU - Suominen, P

AU - Siika-Aho, M

AU - Penttila, M

PY - 1999/5

Y1 - 1999/5

N2 - Crystals of the catalytic core domain of a Trichoderma reesei beta-mannanase belonging to glycoside hydrolase family 5 have been grown by the sitting-drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P2(1), with unit-cell parameters a = 50.0, b = 54.3, c = 60.2 Angstrom, beta = 111.3 degrees, and have a single monomer of mannanase in the asymmetric unit. Native data to 2.0 Angstrom resolution have been collected at room temperature using synchrotron radiation. Data for a platinum derivative have been collected to 1.65 Angstrom at 110 K in a very short time at the CCLRC Daresbury synchrotron source, using a charge-coupled device (CCD) as detector.

AB - Crystals of the catalytic core domain of a Trichoderma reesei beta-mannanase belonging to glycoside hydrolase family 5 have been grown by the sitting-drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P2(1), with unit-cell parameters a = 50.0, b = 54.3, c = 60.2 Angstrom, beta = 111.3 degrees, and have a single monomer of mannanase in the asymmetric unit. Native data to 2.0 Angstrom resolution have been collected at room temperature using synchrotron radiation. Data for a platinum derivative have been collected to 1.65 Angstrom at 110 K in a very short time at the CCLRC Daresbury synchrotron source, using a charge-coupled device (CCD) as detector.

KW - SEQUENCE-BASED CLASSIFICATION

KW - SACCHAROMYCES-CEREVISIAE

KW - CRYSTAL-STRUCTURE

KW - ENDOGLUCANASE

KW - CELLULASE

KW - COMPLEX

KW - DOMAIN

KW - GENE

M3 - Article

SN - 0907-4449

VL - 55

SP - 1058

EP - 1060

JO - Acta Crystallographica. Section D, Biological Crystallography

JF - Acta Crystallographica. Section D, Biological Crystallography

ER -