Crystallization and preliminary X-ray diffraction analysis of levansucrase (LsdA) from Gluconacetobacter diazotrophicus SRT4

C Martinez-Fleites, N Tarbouriech, M Ortiz-Lombardia, E Taylor, A Rodriguez, R Ramirez, L Hernandez, G J Davies

Research output: Contribution to journalArticlepeer-review

Abstract

The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non-denaturing reversed-phase HPLC and was crystallized at room temperature by the hanging- drop vapour-diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 Angstrom under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P22(1)2(1) or P2(1)2(1)2, with unit-cell parameters a = 53.80, b = 119.39, c = 215.10 Angstrom.

Original languageEnglish
Pages (from-to)181-183
Number of pages3
JournalActa Crystallographica. Section D, Biological Crystallography
Volume60
DOIs
Publication statusPublished - Jan 2004

Keywords

  • ENZYMATIC-PROPERTIES
  • BACILLUS-SUBTILIS
  • GENE

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