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Crystallization and preliminary X-ray diffraction of Trypanosoma cruzi dUTPase

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Publication details

JournalActa Crystallographica Section D: Biological Crystallography
DatePublished - Feb 1999
Volume55
Number of pages3
Pages (from-to)528-530
Original languageEnglish

Abstract

Crystals of Trypanosoma cruzi dUTPase have been grown. Two different morphologies are observed, depending on the molecular weight of the PEG used as precipitating agent in the mother liquor, both having a hexagonal unit cell with similar dimensions Complete X-ray diffraction data have been collected to low resolution for one of the forms. The space group is P6(3)22, with unit-cell dimensions a = 134.15, c = 147.05 Angstrom. Peaks in the self-rotation function and the solvent content are consistent with two molecules of dUTPase per asymmetric unit.

    Research areas

  • ESCHERICHIA-COLI, DEOXYURIDINE TRIPHOSPHATASE, CRYSTAL-STRUCTURE, PYROPHOSPHATASE, NUCLEOTIDOHYDROLASE, ENZYME, URACIL, VIRUS, DNA

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