Crystallization of the GTP-dependent transcriptional regulator CodY from Bacillus subtilis

E V  Blagova; V M  Levdikov; K  Tachikawa; A L  Sonenshein; A J  Wilkinson

doi:10.1107/S0907444902018358

Crystallization of the GTP-dependent transcriptional regulator CodY from Bacillus subtilis

E V Blagova, V M Levdikov, K Tachikawa, A L Sonenshein, A J Wilkinson

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

CodY is a GTP sensor that represses transcription of early stationary phase and sporulation genes in Bacillus subtilis. As nutrients become limiting, GTP levels fall and CodY-mediated repression is relieved. Crystals of CodY have been grown in the presence and absence of GTP from sodium citrate buffered solutions containing lithium sulfate and diffraction data have been collected extending to 3.5 Angstrom spacing.

Original language	English
Pages (from-to)	155-157
Number of pages	3
Journal	Acta Crystallographica. Section D, Biological Crystallography
Volume	59
DOIs	https://doi.org/10.1107/S0907444902018358
Publication status	Published - Jan 2003

Keywords

NUTRITIONAL REPRESSION
EXPRESSION
OPERON
DEGRADATION
PROMOTER
PROTEINS

Access to Document

10.1107/S0907444902018358

Cite this

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title = "Crystallization of the GTP-dependent transcriptional regulator CodY from Bacillus subtilis",

abstract = "CodY is a GTP sensor that represses transcription of early stationary phase and sporulation genes in Bacillus subtilis. As nutrients become limiting, GTP levels fall and CodY-mediated repression is relieved. Crystals of CodY have been grown in the presence and absence of GTP from sodium citrate buffered solutions containing lithium sulfate and diffraction data have been collected extending to 3.5 Angstrom spacing.",

keywords = "NUTRITIONAL REPRESSION, EXPRESSION, OPERON, DEGRADATION, PROMOTER, PROTEINS",

author = "Blagova, {E V} and Levdikov, {V M} and K Tachikawa and Sonenshein, {A L} and Wilkinson, {A J}",

year = "2003",

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language = "English",

volume = "59",

pages = "155--157",

journal = "Acta Crystallographica. Section D, Biological Crystallography",

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T1 - Crystallization of the GTP-dependent transcriptional regulator CodY from Bacillus subtilis

AU - Blagova, E V

AU - Levdikov, V M

AU - Tachikawa, K

AU - Sonenshein, A L

AU - Wilkinson, A J

PY - 2003/1

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AB - CodY is a GTP sensor that represses transcription of early stationary phase and sporulation genes in Bacillus subtilis. As nutrients become limiting, GTP levels fall and CodY-mediated repression is relieved. Crystals of CodY have been grown in the presence and absence of GTP from sodium citrate buffered solutions containing lithium sulfate and diffraction data have been collected extending to 3.5 Angstrom spacing.

KW - NUTRITIONAL REPRESSION

KW - EXPRESSION

KW - OPERON

KW - DEGRADATION

KW - PROMOTER

KW - PROTEINS

U2 - 10.1107/S0907444902018358

DO - 10.1107/S0907444902018358

M3 - Article

SN - 0907-4449

VL - 59

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JO - Acta Crystallographica. Section D, Biological Crystallography

JF - Acta Crystallographica. Section D, Biological Crystallography

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