The key response-regulator gene of sporulation, spo0A, has been cloned from Bacillus stearothermophilus and the encoded protein purified. The DNA-binding and phospho-acceptor domains of Spo0A have been prepared by tryptic digestion of the intact protein and subsequently crystallized in forms suitable for X-ray crystallographic studies. The DNA-binding domain has been crystallized in two forms, one of which diffracts X-rays to beyond 2.5 Angstrom spacing. The crystals of the phospho-acceptor domain diffract X-rays beyond 2.0 Angstrom spacing using synchrotron radiation.
|Number of pages
|Acta Crystallographica. Section D, Biological Crystallography
|Published - 3 Mar 1999
Bibliographical noteCopyright © 1999 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?ad0052
- BACILLUS-SUBTILIS SPORULATION