Crystallization of YIoQ, a GTPase of unknown function essential for Bacillus subtilis viability

L  Cladiere; E  Blagova; V M  Levdikov; J A  Brannigan; S J  Seror; A J  Wilkinson

doi:10.1107/S090744490302626X

Crystallization of YIoQ, a GTPase of unknown function essential for Bacillus subtilis viability

L Cladiere, E Blagova, V M Levdikov, J A Brannigan, S J Seror, A J Wilkinson

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

YLoQ is a putative ATP/GTP-binding protein of unknown function identified from the complete sequence of the Bacillus subtilis genome. A gene-knockout programme established that yloQ is one of a set of some 270 indispensable genes for the viability of this organism. Crystals of YloQ have been grown from HEPES-buffered solutions at pH 7.5 containing polyethylene glycol and diffraction data have been collected extending to 2.5 Angstrom spacing.

Original language	English
Pages (from-to)	329-330
Number of pages	2
Journal	Acta Crystallographica. Section D, Biological Crystallography
Volume	60
Issue number	2
DOIs	https://doi.org/10.1107/S090744490302626X
Publication status	Published - Feb 2004

Bibliographical note

Keywords

ESCHERICHIA-COLI
STRATEGY
PROTEIN
KINASE
GENES

Access to Document

10.1107/S090744490302626X

branniganja15.pdf

http://www.iucr.org/cgi-bin/paper?cy5012

Cite this

@article{a83764ad48d94a9ea2867ec4f32b5aa6,

title = "Crystallization of YIoQ, a GTPase of unknown function essential for Bacillus subtilis viability",

abstract = "YLoQ is a putative ATP/GTP-binding protein of unknown function identified from the complete sequence of the Bacillus subtilis genome. A gene-knockout programme established that yloQ is one of a set of some 270 indispensable genes for the viability of this organism. Crystals of YloQ have been grown from HEPES-buffered solutions at pH 7.5 containing polyethylene glycol and diffraction data have been collected extending to 2.5 Angstrom spacing.",

keywords = "ESCHERICHIA-COLI, STRATEGY, PROTEIN, KINASE, GENES",

author = "L Cladiere and E Blagova and Levdikov, {V M} and Brannigan, {J A} and Seror, {S J} and Wilkinson, {A J}",

note = "Copyright {\textcopyright} 2004 International Union of Crystallography - http://www.iucr.org/cgi-bin/paper?cy5012",

year = "2004",

month = feb,

doi = "10.1107/S090744490302626X",

language = "English",

volume = "60",

pages = "329--330",

journal = "Acta Crystallographica. Section D, Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

number = "2",

}

TY - JOUR

T1 - Crystallization of YIoQ, a GTPase of unknown function essential for Bacillus subtilis viability

AU - Cladiere, L

AU - Blagova, E

AU - Levdikov, V M

AU - Brannigan, J A

AU - Seror, S J

AU - Wilkinson, A J

PY - 2004/2

Y1 - 2004/2

N2 - YLoQ is a putative ATP/GTP-binding protein of unknown function identified from the complete sequence of the Bacillus subtilis genome. A gene-knockout programme established that yloQ is one of a set of some 270 indispensable genes for the viability of this organism. Crystals of YloQ have been grown from HEPES-buffered solutions at pH 7.5 containing polyethylene glycol and diffraction data have been collected extending to 2.5 Angstrom spacing.

AB - YLoQ is a putative ATP/GTP-binding protein of unknown function identified from the complete sequence of the Bacillus subtilis genome. A gene-knockout programme established that yloQ is one of a set of some 270 indispensable genes for the viability of this organism. Crystals of YloQ have been grown from HEPES-buffered solutions at pH 7.5 containing polyethylene glycol and diffraction data have been collected extending to 2.5 Angstrom spacing.

KW - ESCHERICHIA-COLI

KW - STRATEGY

KW - PROTEIN

KW - KINASE

KW - GENES

U2 - 10.1107/S090744490302626X

DO - 10.1107/S090744490302626X

M3 - Article

SN - 0907-4449

VL - 60

SP - 329

EP - 330

JO - Acta Crystallographica. Section D, Biological Crystallography

JF - Acta Crystallographica. Section D, Biological Crystallography

IS - 2

ER -