Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM

Soon Wen Hoh*, Tom Burnley, Kevin Cowtan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This work focuses on the use of the existing protein-model-building software Buccaneer to provide structural interpretation of electron cryo-microscopy (cryo-EM) maps. Originally developed for application to X-ray crystallography, the necessary steps to optimise the usage of Buccaneer with cryo-EM maps are shown. This approach has been applied to the data sets of 208 cryo-EM maps with resolutions of better than 4 Å. The results obtained also show an evident improvement in the sequencing step when the initial reference map and model used for crystallographic cases are replaced by a cryo-EM reference. All other necessary changes to settings in Buccaneer are implemented in the model-building pipeline from within the CCP-EM interface (as of version 1.4.0).

Original languageEnglish
Pages (from-to)531-541
Number of pages11
JournalActa crystallographica. Section D, Structural biology
Volume76
Issue numberPart 6
DOIs
Publication statusPublished - 1 Jun 2020

Keywords

  • Buccaneer
  • CCP-EM
  • Collaborative Computational Project for Electron cryo-Microscopy
  • Cryo-EM
  • Model building

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