TY - JOUR
T1 - Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells
AU - Munkley, Jennifer
AU - Copeland, Nikki Alexander
AU - Moignard, Victorial
AU - Knight, John
AU - Greaves, Erin
AU - Ramsbottom, Simon Alexander
AU - Pownall, Betsy
AU - Southgate, Jennifer
AU - Ainscough, Justin
AU - Coverley, Dawn Alison
PY - 2011/4
Y1 - 2011/4
N2 - Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
AB - Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
KW - NUCLEAR MATRIX
KW - cyclin E
UR - http://www.scopus.com/inward/record.url?scp=79954614534&partnerID=8YFLogxK
U2 - 10.1093/nar/gkq1190
DO - 10.1093/nar/gkq1190
M3 - Article
SN - 0305-1048
VL - 39
SP - 2671
EP - 2677
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 7
M1 - PMID: 21109536
ER -