Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Jennifer Munkley, Nikki Alexander Copeland, Victorial Moignard, John Knight, Erin Greaves, Simon Alexander Ramsbottom, Betsy Pownall, Jennifer Southgate, Justin Ainscough, Dawn Alison Coverley

Research output: Contribution to journalArticlepeer-review


Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
Original languageEnglish
Article numberPMID: 21109536
Pages (from-to)2671-2677
Number of pages7
JournalNucleic Acids Research
Issue number7
Early online date24 Nov 2010
Publication statusPublished - Apr 2011


  • cyclin E

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