Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Jennifer Munkley; Nikki Alexander Copeland; Victorial Moignard; John Knight; Erin Greaves; Simon Alexander Ramsbottom; Betsy Pownall; Jennifer Southgate; Justin Ainscough; Dawn Alison Coverley

doi:10.1093/nar/gkq1190

Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Jennifer Munkley, Nikki Alexander Copeland, Victorial Moignard, John Knight, Erin Greaves, Simon Alexander Ramsbottom, Betsy Pownall, Jennifer Southgate, Justin Ainscough, Dawn Alison Coverley

Research output: Contribution to journal › Article › peer-review

Abstract

Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

Original language	English
Article number	PMID: 21109536
Pages (from-to)	2671-2677
Number of pages	7
Journal	Nucleic Acids Research
Volume	39
Issue number	7
Early online date	24 Nov 2010
DOIs	https://doi.org/10.1093/nar/gkq1190
Publication status	Published - Apr 2011

Keywords

NUCLEAR MATRIX
cyclin E

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10.1093/nar/gkq1190

http://nar.oxfordjournals.org/content/39/7/2671.long

Cite this

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title = "Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells",

abstract = "Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.",

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AU - Munkley, Jennifer

AU - Copeland, Nikki Alexander

AU - Moignard, Victorial

AU - Knight, John

AU - Greaves, Erin

AU - Ramsbottom, Simon Alexander

AU - Pownall, Betsy

AU - Southgate, Jennifer

AU - Ainscough, Justin

AU - Coverley, Dawn Alison

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N2 - Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

AB - Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

KW - NUCLEAR MATRIX

KW - cyclin E

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DO - 10.1093/nar/gkq1190

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JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

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M1 - PMID: 21109536

ER -

Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Abstract

Keywords

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